Abstract: Water samples sourced from wells in Akure metropolis was analyzed to determine the E. coli counts. Out of the 12 water samples randomly obtained, 58.33% were observed to be laden with E. coli. The E. coli counts ranged between 10-59 cfu mL-1 which was well above WHO recommended value of no E. coli in 100 mL. The study also revealed that E. coli isolates from the wells have different antibiotic sensitivity pattern. The isolates obtained from Araromi and FUTA junction were less sensitive to the antibiotics while isolates from Ilesa garage and rescue hostel were more sensitive to the different antibiotics. The isolates were found to be more sensitive to norfloxacin (100%, gentamycin (100%, ciprofloxacin (100%), chloramphenicol (85.71%) and teramycin (71.43%).