Abstract: Bacterial strains of Pseudomonas (Ps1, Ps2, Ps3, Ps4, Ps5) Azotobacter (Ab1, Ab2, Ab4, Ab5, Ab6) and Azospirillum (As2, As4, As5, As6, As8) were used to inoculate the seeds of Triticum aestivum Var Inqalab-91. Inoculated and non-inoculated seeds were germinated and grown under field conditions until at maturity. All the bacterial inoculations provoked germination and growth parameters in Triticum aestivum, however Azotobacter strains exhibited more pronounced effect after 7 days of germination such as plant height (55.19%), number of leaves (50.01%) and Pseudomonas strains after 4 months of sowing such as plant height (82.60%), spike length (212.73%) and number of spikes (26.66%), when compared with that of control. All strains increased soluble protein content both after 7 days of germination and at maturity; As6 (64.95%) exhibited maximum increase after 7 days and Ab2 (791.79%) after four months of sowing. Auxin contents were also increased with the inoculation of bacterial strains when compared with that of control however, Ps3 (162.96%) exhibited maximum increase after 7 days and As2 (263.33%) after four months of sowing (at maturity). All strains increased peroxidase activity in Triticum aestivum As6 (109.55%) manifested maximum increase after 7 days and Ps1 (1436.09%) after four months of sowing. Acid phosphates activity was also enhanced with all the bacterial strains however As2 (263.54%) showed maximum increase after 7 days, whereas after four months of sowing Ps4 (146.95%) manifested maximum increase in this parameter. These results demonstrate that these strains can be used as biofertilzer for enhancing biomass as well as yield parameters.