Expression of 1-aminocyclopropane-1-carboxylate Deaminase in Rhizobia Promotes Nodulation and Plant Growth of Clusterbean (Cyamopsis tetragonoloba L.)

Research Article

**Expression of 1-aminocyclopropane-1-carboxylate Deaminase in Rhizobia Promotes Nodulation and Plant Growth of Clusterbean (Cyamopsis tetragonoloba L.)**

Clusterbean (Cyamopsis tetragonoloba L. Taub.) is a commercially important crop but its nodulation status is poor under field conditions. Recently, ACC (1-aminocyclopropane-1-carboxylate) deaminase containing rhizobia have been found to improve nodulation and plant growth in various legumes by lowering the level of ethylene, which acts as inhibitor of nodulation. In this study, 95 isolates of Bradyrhizobium/Rhizobium were obtained from the nodules of clusterbean. These rhizobia were screened for utilization of ACC on Dworkin and Foster’s minimal medium and 46.3% Bradyrhizobium/Rhizobium isolates showed the ability to grow on ACC supplemented plates. Seed inoculation of clusterbean with different Bradyrhizobium/Rhizobium isolates caused significant root and shoot elongation at 5 and 10 days of growth on water agar plates. Maximum root and shoot elongation was observed by ACC utilizing isolate GSA11 followed by ACC non-utilizing isolates GSA114 and GSA115 at 5 and 10 days as compared to control uninoculated seedlings. ACC deaminase containing rhizobial isolates GSA6, GSA11 and GSA110 formed 42-48 nodules per plant and caused 80.7-205.3% gain in plant dry weight in comparison to uninoculated plants in sterilized chillum jar assembly conditions at 60 days of plant growth. Whereas, ACC deaminase lacking bacterial strains GSA114 and GSA115 caused only 35.7 and 3.6% increase in plant dry weight, respectively. These ACC utilizing rhizobial cultures could be further exploited for inoculation of clusterbean under field conditions.

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Aakaknsha Khandelwal and S.S. Sindhu, 2012. Expression of 1-aminocyclopropane-1-carboxylate Deaminase in Rhizobia Promotes Nodulation and Plant Growth of Clusterbean (Cyamopsis tetragonoloba L.). Research Journal of Microbiology, 7: 158-170.

URL: https://scialert.net/abstract/?doi=jm.2012.158.170

Research Journal of Microbiology

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Aakaknsha Khandelwal

S.S. Sindhu

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Research Article

**Expression of 1-aminocyclopropane-1-carboxylate Deaminase in Rhizobia Promotes Nodulation and Plant Growth of Clusterbean (Cyamopsis tetragonoloba L.)**

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