Abstract: Studies assessing the resistance of cowpea genotypes with respect to pest behavior are being developed and the selection of plants with insect resistance characteristics presents itself as an alternative to chemical control. The objective of the present study was to evaluate the behavior of Callosobruchus maculatus fed grains of Vigna unguiculata (L.) Walp (cv. "BR 17 Gurgueia") cultivated with five strains of symbiotic diazotrophic bacteria authorized by the Brazilian Ministry of Agriculture, Livestock and Supply (MAPA) for use as cowpea inoculants (UFLA 42C8; BR 3267; UFLA 0384; INPA 0311B; UFLA 48C5) and two control treatments (S/N, without nitrogen; C/N with nitrogen). Grain samples were infested with adult C. maculatus and maintained in laboratory conditions of 25°C and 12 h photophase. After 10 days of infestation the following insect behavioral and developmental parameters were evaluated: preference, number of grains perforated, number of grains with eggs, survival, emergence, insect weight, relative growth rate, consumption rate, conversion of ingested food, enzymatic activity of amylase, lipase, trypsin and chymotrypsin. The treatments most susceptible to attack by C. maculatus were: control C/N and UFLA 48C5, with greater attractiveness (28 and 23%, respectively) and survival (68 and 65%, respectively), while the most resistant were: INPA 0311B, UFLA 42C8 and control S/N, which reduced survival to 30, 24 and 19%, respectively.