Abstract: Whitefly, Bemisia tabaci (Gennadius) is a serious pest of many economic crops through out the world. Farmers depend highly on insecticide usage to manage this pest. In this study, we investigated the capacity of field collected whitefly populations from different crops to develop resistance against imidacloprid, bifenthrin and fenvalerate by selecting up to 8 generations. Selection pressure was given by exposing the adults to insecticides using the treated leaf discs in the Petri dishes at the dosages sufficient to give 60-80% mortality. After 8th generation, strains selected with these three insecticides exhibited 21.90, 7.12 and 4.13-fold increase in tolerance for the three insecticides, respectively. The realized heritability of insecticide resistance was very high in imidacloprid-selected strains compared to bifenthrin and fenvalerate-selected strains. This indicated a high level of risk in the field populations for the development of resistance to imidacloprid compared to bifenthrin and fenvalerate. The mode of inheritance of insecticide resistance was found to be controlled by nearly completely recessive more than one gene.