Abstract: Background and Objective: wheat is an annual crop that needs cold and long day to transition to flowering that is essential in cultivated cereal to maximize yield. But little is reported in flowering inducing substances on flowering induction in spring wheat. Materials and Methods: Two field experiments were done in the Agric Bot. Dept., Mansoura Univ., Egypt, to assess the role of vernalization (VER) and flowering inducing substances 'FIS' (ascorbic acid 'AsA' and benzyl adenine 'BA') application as well as their interaction on wheat growth, flowering yield and grain quality. Results: Thirty days VER treatment increased plant growth, improved physiological characteristics, induced flowering by modulating plant phytohormone concentration and finally increased yield and grain quality. Exogenous application of 75 mg L–1 AsA plus 50 μM BA under normal or VER treatment increased all studied characters. Conclusion: Application of 75 mg L–1 AsA plus 50 μM BA with 30 days VER three times 30, 40 and 50 days from sowing is suggested to achieve the uppermost growth and yield.