Abstract: The microbial quality of drinking water in the Al-Butana Region has not received much attention from environmentalists. In this study, a total of 100 drinking water samples were collected in sterile 50 mL medical laboratory plastic bottles. The samples were taken from different drinking water sources (1) direct well source (out-let tap water) (2) public reservoirs (3) distribution system (4) common public water stands (locally known as sabeel) (5) house pots (6) house refrigerators (7) donkey driven cart locally known as “karo” and (8) different drinking water utensils: (a) “ibreeg”; (b) drinking jug and (c) hand carrying cooler. Results revealed that bacterial contamination of well drinking water sources is variable depending on the hygienic status and behavior of users. None of the well water samples taken from direct well source showed any positive result of coliform test while 35% of those taken from public water stands (sabeels) showed a positive result of the test. The tests for faecal streptococci were unanimously negative in all of the investigated sites. Coverless reservoirs were found to be the main probable source of coliform bacteria via birds’ feces while handless utensils used normally for drinking are the most source of coliform bacteria in public stands (sabeels).