Abstract: This study highlight the assessment of environmental factors and comfort levels of a ventilated paint shop area. The study was carried out at an automotive components assembly factory. The environment examined was the relative humidity (%), air temperature ( °C) and CO2 (ppm) of the surrounding workstation area. The environmental factors were measured using thermal comfort apparatus, which is capable to measure simultaneously those mentioned environmental factors. The time series data of fluctuating level of factors were plotted to identify the significant changes of factors. Then the thermal comfort of the workers was assessed by using ISO Standard 7730 and thermal sensation scale by using Predicted Mean Vote (PMV). Further Predicted Percentage Dissatisfied (PPD) is used to estimate the thermal comfort satisfaction of the occupant. Finally the PMV and PPD were plotted to present the thermal comfort scenario of workers involved in related workstation. The thermal comfort assessment of this workplace which is warm following by thermal sensation and likely to be dissatisfied by the occupant. Finally, the outcomes of 7 surveys response to the environmental factor questions are discussed.