Abstract: The aim of this study was to examine the discriminatory power of random amplified polymorphic DNA (RAPD) markers in sunflowers, and to determine the effect of various physical exposures on two different sunflower genotypes at molecular level. These exposures are different levels of γ-radiation, magnetic fields and a combination of both. The effect of these exposures on sunflower varieties has been determined using the RAPD method. The results of this study showed that γ-radiation and magnetic fields altered the genetic markers of sunflowers.