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International Journal of Plant Breeding and Genetics
  Year: 2011 | Volume: 5 | Issue: 3 | Page No.: 277-285
DOI: 10.3923/ijpbg.2011.277.285
 
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RAPD Analysis for Detection and Fingerprinting of Sunflower Mutants Induced by Sodium Azide

Gehan G. Mostafa and Asmaa M. Alfrmawy

Abstract:
The aim of this study was to identify and fingerprint the mutants of sunflower plants using RAPD analysis. Ten mutants of two cultivars Giza 1 and Giza 102 of Helianthus annuus produced by sodium azide treatments and the control were evaluated by RAPD markers. The mutants of cultivar Giza 102 were as follows: mutant 1 which changes in the form of inflorescence, mutant 2 is a dwarfed plant, mutant 3 is a plant with large leaves, mutant 4 is a plant with many inflorescences, mutant 5 is a dwarfed plant also. The mutants of cultivar Giza 1 were as follows: mutant 6 with reddish leaf petiole, mutant 7 is a dwarfed plant, mutant 8 with changes in the leaf form, mutant 9 is a plant with dark green inflorescence calyx, mutant 10 is a plant with stripped ray florets. Ten random primers were used; eight out of ten primers used showed variations among the mutants. The 98 amplified fragments were yielded, 83 bands were polymorphic and the total polymorphic rate was 84.6%. Some fragments were common for both genotypes and others were absent as a result of treatments. A dendrogram based on the genetic distance grouped the mutants and the two control cultivars to three clusters A, B and C. The polymorphism detected in all the mutants with both genotypes suggested that sodium azide could be used as effective mutagenic agents in sunflower. RAPD marker is a powerful technique enables fingerprinting of selected mutants in breeding of sunflower plants.
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How to cite this article:

Gehan G. Mostafa and Asmaa M. Alfrmawy, 2011. RAPD Analysis for Detection and Fingerprinting of Sunflower Mutants Induced by Sodium Azide. International Journal of Plant Breeding and Genetics, 5: 277-285.

DOI: 10.3923/ijpbg.2011.277.285

URL: https://scialert.net/abstract/?doi=ijpbg.2011.277.285

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