Abstract: Background and Objective: Tangerine peel has high medical value due to its physiological active including volatile components, flavonoids, alkaloids etc. In this paper, the aim of this work was to study the spectral properties of tangerine peel and the binding interaction between hesperidin of its effective components and bovine serum albumin (BSA). Methodology: The fluorescence spectroscopy was used with the different excitation wavelength under simulative physiological conditions. The dynamic quenching mechanism could be described by the Stern-Volmer equation. The binding parameters between hesperidin and BSA was calculated by double logarithmic equation. Results: The results show the fluorescence peak of tangerine peel is about 448 nm and there a certain red shift occur with the increase of the excitation. The investigation of between BSA and hesperidin show that hesperidin could intact with BSA and the hesperidin-BSA complex was formed. The binding constants between hesperidin and BSA are 3.26, 2.71 and 1.98×104 mol–1 L at 298, 305 and 310 K, respectively, indicating the binding capacity of hesperidin to BSA was weakened with the increasing temperature. Conclusion: It is concluded that, the peak wavelength of tangerine peel is about 448 nm, the hesperidin could interact with BSA, the fluorescence quenching of BSA caused by hesperidin is a static quenching.