Abstract: Some biochemical and histological effects of consumption of Musa paradisiaca-supplemented diet in hepatotoxic rats were investigated. Twenty-four rats were divided into four hepatotoxic and non-hepatotoxic groups and fed a Musa paradisiaca-supplemented diet. The parameters measured included alanine transaminase, aspartate transaminase, total protein, glucose, total triglycerides, total cholesterol, reduced glutathione, lipid peroxidation and packed cell volume. Histological changes in tissue sections of liver and testes were also examined. The results obtained showed that alanine transaminase and aspartate transaminase did not significantly change except in the hepatotoxic control group which showed an increase in aspartate transaminase. Cholesterol and triglycerides were significantly (p<0.05) increased in the hepatotoxic group fed Musa paradisiaca-supplemented diet. Protein and reduced glutathione levels were significantly (p<0.05) increased in non-hepatotoxic rats fed Musa paradisiaca-supplemented. Lipid peroxidation, glucose and PCV levels were not significantly altered in all the groups. The consumption of a Musa paradisiaca-supplemented diet did not significantly change the weight of the animals. Histological observations of tissue sections of liver showed necrosis in the hepatotoxic rats and varying regeneration in the groups fed Musa paradisiaca-supplemented diet while there were no changes in the histology of the testes in all the groups.