Abstract: A polyherbal decoction prepared with Gongronema latifolia, Ocimum gratissimum and Vernonia amygdalina leaves was evaluated for analgesic and anti inflammatory activities using rats and mice. Three doses of the decoction (2.0, 4.0 and 8.0 g kg-1) were administered to the test animals to evaluate analgesic and anti inflammatory activities using the test models of formalin-induced pain, mouse writhing, hot plate, carrageenan and xylene induced oedema. Acute toxicity studies showed that the extract may be considered non toxic as no mortality was observed even at the high doses of 16 g kg-1 p.o. and 2.5 g kg-1 i.p. In the hot plate assay, the decoction caused a significant pain inhibition of 138.5% (12.4±1.9) which is comparable with 180.8% produced by morphine. The decoction, at 8.0 g kg-1 b.wt., significantly inhibited writhing by 138.5% (12.4±1.9) when compared to that of control. A dose-dependent decrease in licking time and frequency in rats injected with 1% formalin was observed. The highest dose of the decoction exhibited a significant inhibition of 46% (42±5.4) while acetylsalicylic acid also produced a significant inhibition of 49% (39.6±2.9) for the formalin induced pain assay. The decoction also significantly (p<0.05) reduced carrageenan and xylene induced oedema. These results suggest that the polyherbal decoction which is used as a tonic in folk medicine has the potential to reduce pain and inflammation and is also, non toxic.