Abstract: The aim of this study is the evaluation of the antibacterial activity of cumin essential oil on burn isolates of P. aeruginosa. Fifty two burn isolates of P. aeruginosa were obtained from burn wards of two hospitals at Tehran, Iran. The susceptibility of isolates was determined using a broth microdilution method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of isolates to cumin essential oil was determined. The susceptibilities of isolates to different antibiotics were tested using agar disk diffusion method. The rates of resistances were determined to antibiotics as follows: Gentamicin 96%, ceftazidime 100%, tobramycin 100%, kanamycin 100%, amikacin 73%, ceftizoxime 100%, piperacillin 94.2%, imipenem 50% and ciprofloxacin 71%. Cumin essential oils possessed antibacterial effect against all isolates of P. aeruginosa, with MIC and MBC values in the range of 0.015 to 0. 25 ml mL-1. These results suggest the potential use of the cumin essential oil for the control of P. aeruginosa infections.