Abstract: Genetic variability study generates very relevant information on the possibility of genetic improvement of crops for yield and quality attributes. The study was objectively designed to assess the genetic variability of thirty six ginger (Z. officinale Rosc.) accessions. The experiment was laid out in a 6x6 simple lattice design with two replications during 2009-2010 main cropping seasons at two locations i.e., Tepi and Bahir Dar. Variances component method was used to estimate genetic variation, broad sense heritability and genetic advance. Number of plants per plot, fresh rhizome yield and dry rhizome yield showed high Genetic Coefficient of Variation (GCV) and Phenotypic Coefficient of Variation (PCV) at both locations. Relatively high heritability and genetic advance was obtained for oleoresin content, volatile oil content, fiber content, fresh rhizome yield and dry rhizome yield. The D2 analysis showed the 36 genotypes grouped into 7 and 11 clusters at Tepi and Bahir Dar, respectively. This makes the genotypes to become highly divergent. The overall assessment showed that there is wide variability among ginger accessions in Ethiopia which has an important implication for breeding ginger for yield, morphological and quality attributes.