Abstract: Investigation of antagonistic activity of selected Pseudomonads bacterial strains against Xanthomonas axonopodis pv. citri the causal agent of citrus bacterial canker disease was carried out in the laboratory and greenhouse conditions. Results of in vitro experiments showed that all bacterial strains were inhibitory on X. citri with various antagonistic activities. The most effective strains were selected and were evaluated in the greenhouse against citrus canker disease. Results of greenhouse experiments in application of antagonistic bacterial strains to control X. axonopodis pv. citri the causal agent of citrus bacterial canker disease were promising and selected strains reduced the number of disease spots between 23.8 to 64.0%.