Abstract: A survey was carried out to determine the seroprevalence of avian influenza (AI) in poultry from 12 Local Government Areas (LGAs) of Kogi state. A total of 779 sera were analyzed for AI (H5) antibodies by haemagglutination inhibition test with overall seroprevalence of 4.2% and mean antibody titre of 8.42±0.254 log2. The seroprevalence of AI (H5) among the LGAs was highest in Kabba/Bunu LGA with 12.3% while it was zero in Ajaokuta and Okene LGAs. The seroprevalence of AI (H5) in the sampling units was highest for backyard poultry with 4.5% and lowest for rural poultry with 3.8%. Using enzyme link immunosorbent assay, the same sera were analyzed for AI nucleoprotein antibodies with overall seroprevalence of 30.8%. The seroprevalence of AI nucleoprotein in the LGAs was highest for Mopamuro LGA with 41.8% and lowest for Adavi LGA with 18.6% while among the poultry species it was highest in chicken with 31.6% and zero in duck. Also, among the sampling units the seroprevalence of AI nucleoprotein was highest in rural poultry with 35.0% and lowest in backyard poultry with 26.5%. The presence of subtype specific (H5) and type A influenza antibodies in poultry in the surveyed area indicated a previous exposure to the AI viruses. The detection of AI (H5) antibodies was more in backyard poultry and might be due to vaccination against HPAI (H5N1) by poultry farmers in Kogi state. It is recommended that further surveillance for AIVs and subtype characterization of AIVs should be carried out.