Abstract: Background and Objective: The regulatory gamma subunits of AMP-activated protein kinase (AMPK) are encoded by the 5'-AMP-activated protein kinase gamma subunit (PRKAG) genes. These genes are dominantly expressed in skeletal muscle and many reports of the animal counterparts suggest that these subunit genes play key roles in the regulation of energy metabolism in the skeletal muscle tissues. This study was designed to identify and analyze human and avian PRKAG genes in a genome scale. Materials and Methods: In the study, all the putative PRKAG gene sequences were used to construct ML (maximum likelihood) phylogenetic trees with package MEGA version 6.06 under JTT+I+G model. After phylogenetic analyses, the putative PRKAG gene were further subjected to the enrichment analyses of gene ontology (GO) and pathway annotations. In this study, there were totally 58 putative PRKAG genes and 31 unique PRKAG genes identified from the human and avian genomes. Results: Phylogenetic analyses indicated that among all the three sub-families of PRKAG genes (i.e. PRKAG1, PRKAG2 and PRKAG3), those protein sequences of PRKAG genes from four avian genomes formed monotonous phylogenetic clusters, whereas all the human protein sequences of PRKAG genes formed sole phylogenetic clusters. Furthermore, functional enrichment analyses of GO and pathway annotations revealed that PRKAG genes were functionally enriched in energy metabolism related signaling pathways and biological processes with significant p-values observed. Conclusion: The dataset and results of this study will facilitate further study on PRKAG genes in domestic animals.