Abstract: A study was conducted to know the effectiveness of Randomly Amplified Polymorphic DNA (RAPD) marker in generating polymorphism in different chicken populations. Four of the twenty random primer screened yielded distinct RAPD profiles. Among 39 fragments amplified from these 4 primers, 25 of them showed polymorphism. The average number of amplified bands per primer ranged from 9 to 11. The study revealed that RAPD markers were effective in detecting polymorphism in different chicken breeds. However, comparatively large numbers of random primers were required to detect satisfactory polymorphism in different chicken populations.