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  Year: 2010 | Volume: 9 | Issue: 2 | Page No.: 170-175
DOI: 10.3923/biotech.2010.170.175
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Identification of Genomic Markers by RAPD-PCR Primer in Leukemia Patients

N. Saleh, M.A. Ibrahim, E. Archoukieh, A. Makkiya, M. Al-Obaidi and H. Alobydi

The aim of this study is to ascertain the possible application of Random Amplification of Polymorphic DNA (RAPD) analysis as a genetic test to investigate DNA polymorphisms and detection of genomic markers in various types of leukemia. The results showed unique profiles of amplified DNA fragments produced in genomic DNA of three types of leukemia by an arbitrary primer of decamer oligonucleotides OPA-09. The primer produced four types of amplified DNA fragments (980, 1659, 2187 and 3162 bp). The smallest amplified DNA fragment (980 bp) appeared in 14.3 and 13.3% of tested acute myeloid leukemia and chronic myeloid leukemia patients, respectively; but was absent in genomic DNA of chronic lymphoid leukemia and normal individuals. Whereas the largest amplified fragment (3162 bp) was present in 12.5, 20 and 75% of chronic lymphoid leukemia, chronic myeloid leukemia and normal individuals, respectively and was absent in acute myeloid leukemia. On the other hand, the two amplified fragments (1659 and 2187 bp) were present in normal and leukemia patients. Cluster analysis of amplified DNA fragments grouped the leukemia patients in two main groups. The detected DNA polymorphisms by the arbitrary primer OPA-09 might find application in developing efficient RAPD primer for diagnosis of leukemia.
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  •    Molecular Diagnostics and Therapy of Acute Myeloid Leukemia
  •    Perspective of DNA Methylation in Cancer Research
  •    Prospects for DNA Methylation Research in Psychiatric Disorders
  •    Advances in Genomic DNA Methylation Analysis
  •    Molecular Characterization of Pomegranate (Punica granatum L.) Landraces Grown in Jordan using Amplified Fragment Length Polymorphism Markers
  •    Analysis of Genetic Diversity in Bangladeshi Chicken using RAPD Markers
  •    Effectiveness of RAPD Marker in Generating Polymorphism in Different Chicken Population
How to cite this article:

N. Saleh, M.A. Ibrahim, E. Archoukieh, A. Makkiya, M. Al-Obaidi and H. Alobydi, 2010. Identification of Genomic Markers by RAPD-PCR Primer in Leukemia Patients. Biotechnology, 9: 170-175.

DOI: 10.3923/biotech.2010.170.175






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