Abstract: An experiment was conducted to determine the influence of organic acid salts and mannan oligosaccharides on the performance and gut health of Japanese Quail. Day old chicks of Japanese Quail (n = 280) were randomly assigned into seven dietary treatments replicated four times with 10 chicks per replicate. Control (C0) birds were given a standard basal diet, C1 birds were provided with basal diet having antibiotic (Bacitracin methyl disalisylate-BMD) at 0.5 g kg-1; T1 birds having diet with sodium butyrate at 5 g kg-1; T2 birds were provided with basal diet having Mannan Oligosaccharide (MOS) at 1 g kg-1; group T3 birds were fed basal diet with ammonium formate at 1 g kg-1, calcium propionate 1 g kg-1 and sodium butyrate 5 g kg-1; T4 birds having diet with sodium butyrate at 5 g kg-1 and MOS at 1 g kg-1; T5 birds having diet with ammonium formate at 1 g kg-1, calcium propionate at 1 g kg-1, sodium butyrate at 5 g kg-1 and MOS at 1 g kg -1 of the diet. Organic acid salt supplementation increased (p<0.05) live weight, live weight gain compared to control (C). Cumulative feed intake was not significantly affected due to dietary treatments. Superior results in terms of Feed Conversion Ratio (FCR) and Performance Index (PI) were found in organic acid salt combination (T3) and organic acid salts combination (OASC) with MOS (T5) supplemented groups compared to others. OASC and OASC with MOS improved (p<0.05) gut health by reducing bacterial load in gut compared to control (C0) and other groups also. The result showed OASC and OASC with MOS supplementation significantly improved villus height compared to other groups. This experiment revealed that the use of OASC or OASC with MOS in the diet improved body weight gain, FCR, PI, villus height and reduced bacterial load in gut as antibiotic compared.