Abstract: The aim of the present investigation is to identify a suitable and cost efficient substitute of Gibberellic acid to enhance hybrid rice seed production. The experimental material comprising both the parental lines of hybrid NDHR2 i.e., IR58025A and NDR3026-3-1R were treated with 34 treatment combination of different growth regulators and one untreated check were grown in randomized block design with three replications. The results indicated that the analysis of variance was significant for all the characters except plant height and effective tillers per plant. The exogenous application of various plant growth hormones significantly increases seed yield with a range of 14.85 g to 23.54 g. Treatment T26 (GA3 45g + C.C.) had highest significant increase in grain yield followed by T2 (GA3 45 g), T1 (GA3 30 g), T5 (NAA 200 g), T3 (NAA 100 g), T27 (Urea 2 g + C.C.) and T24 (GA3 45 g + K2PO4 2 g). The other yield components were also significantly increases with the foliar application of growth regulators. It is concluded that treatment combination T26 (GA3 45 g+ Urea 10g + Boric acid 2 g + ZnSO4 + K2 PO4 2 g) gave best results and could be used to enhance hybrid rice seed production and substitute of GA3 in India as well as other hybrid rice growing countries.