Abstract: In this protocol two primers were tested against the extracted DNA, where all the amplified genotypes were found to be similar against primer AR01. The reaction of all the genotypes under observation exhibited a series of variations against primer AR02. The plants analyzed cytologically exhibited a regular mitotic behaviour except variety sargab-92, in which 2n= 40 and 41 chromosomes have been reported. All other varieties were proved to be hexaploid with 2n= 42. High molecular weight (HMW) glutenin subunits from 7 hexaploid wheat genotypes were also fractionated by SDS-PAGE, in order to characterise the material under observation. In this experiment 6 genotypes were found to be identical, while a great variation was observed within the variety sargab-92, where some extra bands have been reported.