Abstract: The aim of the present study was to investigate the influence of Pendimethalin (PM) on growth and anatomical characteristics of Cucumis sativus and Echinochloa crus-galli plants. Moreover, Psudomonas putida and compost were evaluated for detoxification of PM in soil. Seeds were sown in pots containing either PM treated soil or PM and P. putida or compost treated soil. Twenty eight days later, the phytotoxicity bioassay was performed on the growing plants. PM significantly decreased germination and increased seedling mortality percentages. Cotyledonary leaf and hypocotyls and true leaves of C. sativus seedling treated with PM seemed to be dark green colors, swelling and brittleness. In addition, reduction in fresh and dry weights of the treated plants was observed. In contrary, chlorophyll contents were significantly increased. Reducing in number of xylem vessels was found not only in hypocotyl but also in the first true leaf of C. sativus plants. Insignificant differences were observed in the most growth and anatomical parameters between P. putida or compost applying in PM contaminated soil comparing with PM treated plants. P. putida and compost were effective in PM degradation in soil with half-life of 4.67 and 5 days, respectively. PM half-life was 51.9 days in untreated clay soil. The results suggested that bioremediation by P. putida and compost was proved as an effective method for detoxification of pendimethalin in soil.