Abstract: In the present study an attempt has been made to assess the genotypic variability among twelve species of the genus Tephrosia, distributed in Andhra Pradesh, through DNA fingerprinting using RAPD technique. Twenty OPC (Operon Biotechnologies GmbH, Germany) primers were used. The cluster analysis based on the similarity matrix was performed using the Unweighted Pair Group Method with Arithmetic Average (UPGMA) with the help of PHYLIP software ver. 3.65 pooled from all the six primers. Present study has justified to a great extent co-relating with the classification based on the morphological traits. However a distinction between some members of the genus Tephrosia is still a matter of debate. Hence further analyses are needed to determine the correct intrageneric taxonomic treatment of Tephrosia, since it represents one of the largest and most complex groups in the core tribe Millettieae of the family Fabaceae. This study represents the first approach in using nuclear DNA finger print markers as a tool to study molecular systematics of the genus Tephrosia.