Abstract: Objective: This study was conducted to evaluate the toxicity, developmental and morphological effects of eight insecticides related to different groups, two plant crude extracts and three plant volatile oils, against Musca domestica (M. domestica) 2nd instar larvae using 3 treatment techniques. Methodology: Experiments were conducted in Toxicology Laboratory, Faculty of Agriculture, Menoufia University. The insecticides as applied as mixed with food media, residual film and dipping techniques. The data were statistically analyzed using one way ANOVA by F test at LSD 5% probability. Results: The dipping technique was the most effective one. Methomyl was the most toxic compound recording at LC50 4.93 ppm after 24 h of treatment. Citronella oil was the most toxic one recorded LC50 2663.91 ppm. Khaya crude extract was the most toxic one, recording LC50 3300.25 ppm compared with other plant volatile oils and extracts. Chlorantraniliprole and spinosad showed higher pupicidal activity compared with other tested substances. Nearly all tested substances decreased larval, pupal and adult numbers and duration by three methods. Larval, pupal and adult survival percentages were reduced. Adult survival percentages were sharply decreased in three treated techniques, respectively. There were different forms of larval, pupal and adult abnormalities after treated with sublethal concentartions of tested compounds by three methods. Conclusion: The dipping technique is most effective method for control larval stage of house fly, selected insecticides shows more toxicity compared with other tested compounds and tested insecticides, plant volatile oils and plant extracts induced biological and morphological effects.