Abstract: Health damaging effects of carbonated soft drink are largely based on epidemiological observations. The present study aimed to examine the possible physiological and biochemical effects of such drink in animal. Through oxiblot assay, fluorescence activated cell sorting assay and other biochemical investigations; it has been shown that consumption of such drink makes oxidative damages. Extensive such damages at the level of proteins in vivo in the liver, kidney and blood of experimental animal, guinea pigs, which cannot synthesize vitamin C like humans have been observed. These effects are also associated with significant cellular damages. In vitro studies reviled that such drink contains oxidants those are capable of direct protein oxidation. These observations establish physiological and biochemical link between consumption of carbonated soft drink and its deleterious effects in vitamin C deficient animals.