Abstract: The purpose of this study was to evaluate the influence of Hyponidd, a herbomineral formulation and its effect on key enzymes of glucose metabolism and lipid metabolism in STZ-induced diabetic rats. Female Wistar rats with a body weight of 180-200 g were used in this study. The rats were divided into seven groups of six rats each after the induction of STZ-diabetes: normal rats; normal rats given Hyponidd (200 mg kg 1 b. wt.); diabetic control; diabetic rats given Hyponidd (50 mg kg 1 b. wt.); diabetic rats given Hyponidd (100 mg kg 1 b. wt.); diabetic rats given Hyponidd (200 mg kg 1 b. wt.); diabetic rats given glibenclamide (600 µg kg 1 b. wt.). After 45 days of treatment, fasting blood glucose, plasma insulin, serum and tissue carbohydrate metabolizing enzymes and lipid profiles were determined in normal and streptozotocin induced-diabetic rats. Oral administration of Hyponidd for 45 days resulted in significant (p<0.05) reduction in blood glucose, serum and tissue glucose-6-phosphatase, fructose-1, 6-bis phosphatase, total cholesterol, triglyceride and free fatty acids level. At the same time there was a significant increase in the levels of plasma insulin, hexokinase and HDL cholesterol in streptozotocin induced-diabetic rats. The effect of Hyponidd was compared with an oral hypoglycaemic agent, glibenclamide. Present study shows, that Hyponidd significantly restored the altered carbohydrate and lipid metabolism by exerting a beneficial action against secondary complications associated with diabetes mellitus.