Abstract: Fibroblast Growth Factor (FGF) family is considered as local cytokines produced from many physiological systems including ovary. The FGFs regulate many cellular processes like angiogenesis, cell proliferation, cell survival, organogenesis and embryonic development including ovarian folliculogenesis. Fibroblast Growth Factor 10 (FGF10) is one of most important member in FGF family which mostly mediates epithelial cell motility, differentiation, migration and wound healing in many cellular system. The FGF10 is also known to regulate the ovarian follicular dynamics in an autocrine and paracrine manner. Therefore, the present study was undertaken aimed with an objective to evaluate the expression and localization of FGF10 in different follicular size or groups during various stages of follicular development in buffalo ovary. The mRNA and protein expression of FGF10 transcripts did not differ significantly in all follicular size during follicular development. The FGF10 was exclusively localized in Granulosa Cell (GC) and Theca Interna (TI) showing a stage specific immunoreactivity throughout the follicular developmental process in buffalo ovary. The present findings suggest the species specific expression and localization pattern of FGF10 in buffalo ovarian follicle.