Abstract: The present study was undertaken to evaluate the hepato-protective effect of crude flavonoid fraction of aerial parts of Lippia nodiflora in ethanol induced oxidative stress in liver using experimental animal models. Ethanol-fed (5 g kg-1 day-1) male rats were treated by crude flavonoid fraction (25, 50 mg kg-1) for 21 days. The liver damage was indicated by the significant increase in serum AST, ALT, ALP, LDH, total bilirubin, urea and decrease in total protein and triglyceride (TG). Lipid peroxidation markers like Thiobarbituric Acid Reactive Substances (TBARS) and antioxidant enzymes namely superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), Glutathione Reductase (GR) and Glutathione-S-transferase (GST) activities were also measured. The crude flavonoid fraction showed significant (p<0.05) protective effect by decreasing the elevated liver marker enzymes, total bilirubin, lipid peroxidation marker and ameliorated the diminished serum total protein as well as antioxidant levels in a dose dependent manner. The protective effect of the crude flavonoid fraction was observed at both concentrations and was compared to that of the standard used. Hence, our results highlights the ability of crude flavonoid fraction of Lippia nodiflora to ameliorate oxidative damage in the liver and the observed effects are associated with its antioxidant activities.