Abstract: Goat’s industry becomes very attractive in various countries and play a vital role in socio-economic organization. A different diet causes different effects in the animal performance. Therefore, this study was intended to detect the effect of different diets on the number and distribution of endocrine Gastrin (G) cells in the goat’s abomasum. Twenty four, 9 months aged local goats were equally divided into control and two treatment groups. The control group was fed a diet consisting of 60% guinea grass and 40% concentrates consist of corn, soya bean meal, calcium, minerals, vitamins and 12.8% crude protein. The first treatment group was fed with 10% guinea grass and 90% palm kernel cake consist of calcium, minerals, vitamins and 15.2% crude protein. The second treatment group was fed with a diet similar to those of the first treatment group but with addition of molybdenum 40 ppm and sulphur 100 ppm. Goats in each group were fed daily with the selected diet for 120 days and then slaughtered and their abdomens were dissected and the abomasums were removed and processed for both histological and immunohistochemistry standard procedures. Microscopic findings revealed that treated groups that fed with high protein diet showed significantly (p<0.05) higher number of G cells than those of the control goats. Fluorescein isothiocyanate technique showed G cells distributed only in the pyloric mucosa of the abomasum with specific pattern of distribution. Current data concluded that G cells density in the pyloric mucosa have different responses to the different supplemented diets.