• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Biotechnology
  2. Vol 9 (1), 2010
  3. 17-24
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Biotechnology

Year: 2010 | Volume: 9 | Issue: 1 | Page No.: 17-24
DOI: 10.3923/biotech.2010.17.24

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 360

Search


Authors


P. Abdeshahian

Country: Malaysia

N. Samat

Country: Malaysia

W.M. Wan Yusoff

Country: Malaysia

Keywords


  • Palm kernel cake
  • b-glucosidase
  • Aspergillus niger
  • solid substrate fermentation
  • column bioreactor
Research Article

Utilization of Palm Kernel Cake for Production of β-Glucosidase by Aspergillus niger FTCC 5003 in Solid Substrate Fermentation Using an Aerated Column Bioreactor

P. Abdeshahian, N. Samat and W.M. Wan Yusoff
The production of β-glucosidase by Aspergillus niger FTCC 5003 from palm kernel cake as a substrate was studied in solid substrate fermentation using a laboratory column bioreactor. The simultaneous effects of three independent variables, namely incubation temperature, initial moisture content of substrate and airflow rate on the production of β-glucosidase were evaluated using response surface methodology on the basis of a central composite face-centered design. A total of 18 experiments were carried out in which Aspergillus niger FTCC 5003 was cultivated on PKC in an aerated column bioreactor for 7 days under incubation temperature, moisture level and aeration rate determined. Experimental results showed that the highest activity of β-glucosidase (52.06 U g-1) was obtained at an incubation temperature, an initial moisture level and an aeration rate of 32.5°C, 60% and 1.5 L min-1, respectively. Statistical analysis revealed that the quadratic terms of incubation temperature and initial moisture content had highly significant effects on the production of β-glucosidase (p<0.01). The statistical results also showed that the interaction effect between initial moisture level and aeration rate significantly influenced the production of β-glucosidase (p<0.05). Optimum conditions suggested by the second-order polynomial regression model for attaining maximum β-glucosidase production were 31.6°C incubation temperature, 57.0% initial moisture and 0.5 L min-1 aeration rate with a predicted production value of 47.20 U g-1.
PDF Fulltext XML References Citation

How to cite this article

P. Abdeshahian, N. Samat and W.M. Wan Yusoff, 2010. Utilization of Palm Kernel Cake for Production of β-Glucosidase by Aspergillus niger FTCC 5003 in Solid Substrate Fermentation Using an Aerated Column Bioreactor. Biotechnology, 9: 17-24.

DOI: 10.3923/biotech.2010.17.24

URL: https://scialert.net/abstract/?doi=biotech.2010.17.24

Related Articles

Effect of Different Diets on Number and Distribution of Gastrin Cells in the Mucosa of Goat’s Abomasums
Production of Biofuel using Biomass as a Sustainable Biological Resource

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved