R. Baksha
Breeding Division, Bangladesh Sugarcane Research Institute, lshurdi-6620,
Pabna, Bangladesh
R. Alam
Breeding Division, Bangladesh Sugarcane Research Institute, lshurdi-6620,
Pabna, Bangladesh
M.Z. Karim
Breeding Division, Bangladesh Sugarcane Research Institute, lshurdi-6620,
Pabna, Bangladesh
Sk. A. Mannan
Breeding Division, Bangladesh Sugarcane Research Institute, lshurdi-6620,
Pabna, Bangladesh
B.P. Podder
Breeding Division, Bangladesh Sugarcane Research Institute, lshurdi-6620,
Pabna, Bangladesh
A.B.M.M. Rahman
Breeding Division, Bangladesh Sugarcane Research Institute, lshurdi-6620,
Pabna, Bangladesh
ABSTRACT
Microshoots were cultured on MS medium supplemented with different combinations of auxin, sucrose and pH level for in vitro rooting of callus induced sugarcane (Saccharum officinarum) variety namely Isd 31. Among the types and concentrations of auxin used the best rooting in all the microshoots was obtained with half strength (major salts) MS basal medium supplemented with NAA at 5.0 mg l‾1, sucrose at 50 g l‾1 and pH level 5.7 were the optimum conditions for rooting when microshoots were incubated 25+ 2°C under 16 h photo period regime. The regenerated plantlets were successfully transferred to soil and the percentage of survivability under ex vitro conditions was 75.
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How to cite this article
R. Baksha, R. Alam, M.Z. Karim, Sk. A. Mannan, B.P. Podder and A.B.M.M. Rahman, 2003. Effect of Auxin, Sucrose and pH Level on in vitro Rooting of Callus Induced Micro
Shoots of Sugarcane (Saccharum officinarum). Journal of Biological Sciences, 3: 915-920.
DOI: 10.3923/jbs.2003.915.920
URL: https://scialert.net/abstract/?doi=jbs.2003.915.920
DOI: 10.3923/jbs.2003.915.920
URL: https://scialert.net/abstract/?doi=jbs.2003.915.920
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