In vitro Shoot Tip Culture of Sugar-cane (Saccharum officinarum) Variety Isd 28

Research Article

In vitro Shoot Tip Culture of Sugar-cane (Saccharum officinarum) Variety Isd 28

R. Baksha, R. Alam, M.Z. Karim, S.K. Paul, M.A. Hossain, M.A.S. Miah and A.B.M.M. Rahman

Multiple shoots were obtained from shoot tip explant of sugar-cane (Saccharum officinarum) cultured on MS medium supplemented with BAP (0.5-2.0 mg l^-1), Kn (0.1-0.5 mg l^-1) and IBA (0.1-0.5 mg l^-1). Roots were induced in in vitro regenerated shoots on half MS medium supplemented with 5.0 mg l^-1 NAA, IBA and IAA. Plant regeneration from shoot tip was the highest on MS medium supplemented with BAP 2.0 mg l^-1 and IBA 0.5 mg l^-1. The reported experimental findings present a method of plant regeneration of sugar-cane variety Isd 28 through shoot tip culture. The plantlets were successfully transferred to soil and the percentage of survivability under ex vitro condition was 70.

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R. Baksha, R. Alam, M.Z. Karim, S.K. Paul, M.A. Hossain, M.A.S. Miah and A.B.M.M. Rahman, 2002. In vitro Shoot Tip Culture of Sugar-cane (Saccharum officinarum) Variety Isd 28. Biotechnology, 1: 67-72.

DOI: 10.3923/biotech.2002.67.72

URL: https://scialert.net/abstract/?doi=biotech.2002.67.72

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Authors

R. Baksha

R. Alam

M.Z. Karim

S.K. Paul

M.A. Hossain

M.A.S. Miah

A.B.M.M. Rahman

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Research Article

In vitro Shoot Tip Culture of Sugar-cane (Saccharum officinarum) Variety Isd 28

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