B. Santoso
Department of Animal Nutrition,
M.N. Lekitoo
Department of Animal Nutrition,
B. Tj. Hariadi
Department of Animal Nutrition,
T.W. Widayati
Department of Animal Production,
H. Abubakar
Department of Biology, University of Papua, Manokwari, West Papua, 98314, Indonesia
ABSTRACT
The main feed for ruminants is forage, which is composed of polysaccharides. Feed digestion in the rumen is mediated by microbes, thus the type and makeup of rumen microbes are an important factor that affects nutrient digestibility. This study aimed to evaluate the in vitro nutrient digestibility and fermentation characteristics of king grass combined with a concentrate that contained mixed microbes. Lactobacillus plantarum, Saccharomyces cerevisiae and two strains of cellulolytic bacteria (i.e., Acinetobacter baumannii and Pseudomonas aeruginosa) were added to the concentrate. Cellulolytic bacteria were isolated from waste from either rice straw or palm oil seeds. The concentrate was mainly composed of agricultural and food industry wastes, such as cassava waste, tofu waste and rice bran. The following four concentrates were made: A, concentrate without microbe; B, concentrate containing L. plantarum and S. cerevisiae; C, concentrate containing L. plantarum, S. cerevisiae and P. aeruginosa and D, concentrate containing L. plantarum, S. cerevisiae and A. baumannii. Bacteria and yeast were added to the concentrate at 106-107 cfu/g. In vitro nutrient digestibility assays were conducted using 250 mg substrate composed of king grass and concentrate (70: 30, DM). Our findings revealed that concentrate contained 7.2 × 106 cfu/g L. plantarum, 3 × 108 cfu/g S. cerevisiae and 8.6 × 107 cfu/g A. baumannii and P. aeruginosa. The OM digestibility was greater (p<0.01) for the grass substrate with concentrate containing L. plantarum, S. cerevisiae and cellulolytic bacteria than for the concentrate that only contained L. plantarum and S. cerevisiae. Moreover, NDF digestibility was greater (p<0.01) for the grass substrate combined with concentrate that contained mixed microbes compared with the concentrate without microbes. The addition of cellulolytic bacteria increased NH3-N and acetic acid concentrations (p<0.05). We concluded that the addition of mixed microbes to the concentrate improved fermentation activity and the digestibility of nutrients in vitro.
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How to cite this article
B. Santoso, M.N. Lekitoo, B. Tj. Hariadi, T.W. Widayati and H. Abubakar, 2016. In vitro Nutrient Digestibility and Fermentation Characteristics of King Grass Combined with Concentrate-Containing Mixed Microbes. Pakistan Journal of Nutrition, 15: 784-788.
DOI: 10.3923/pjn.2016.784.788
URL: https://scialert.net/abstract/?doi=pjn.2016.784.788
DOI: 10.3923/pjn.2016.784.788
URL: https://scialert.net/abstract/?doi=pjn.2016.784.788
REFERENCES
- Abdulrazak, S.A., T. Fujihara, J.K. Ondiek and E. Orskov, 2000. Nutritive evaluation of some Acacia tree leaves from Kenya. Anim. Feed Sci. Technol., 85: 89-98.
CrossRefDirect Link - Beuvink, J.M.W. and S.F. Spoelstra, 1992. Interactions between substrate, fermentation end-products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen microorganisms in vitro. Applied Microbiol. Biotechnol., 37: 505-509.
CrossRefDirect Link - Chaucheyras, F., G. Fonty, G. Bertin, J.M. Salmon and P. Gouet, 1996. Effects of a strain of Saccharomyces cerevisiae (Levucell SC1), a microbial additive for ruminants, on lactate metabolism in vitro. Can. J. Microbiol., 42: 927-933.
PubMedDirect Link - Fuller, R., 1989. Probiotics in man and animals. J. Applied Bacteriol., 66: 365-378.
CrossRefPubMedDirect Link - Hariadi, B.T. and B. Santoso, 2010. Evaluation of tropical plants containing tannin on in vitro methanogenesis and fermentation parameters using rumen fluid. J. Sci. Food Agric., 90: 456-461.
CrossRefPubMedDirect Link - Hong, H.A., L.H. Duc and S.M. Cutting, 2005. The use of bacterial spore formers as probiotics. FEMS Microbiol. Rev., 29: 813-835.
CrossRefPubMedDirect Link - Krisnan, R., B. Haryanto and K.G. Wiryawan, 2009. The effect of combined probiotics with catalyst supplementation on digestion and rumen characteristic in Priangan sheep. Indonesian J. Anim. Vet. Sci., 14: 262-269.
Direct Link - Lila, Z.A., N. Mohammed, T. Yasui, Y. Kurokawa, S. Kanda and H. Itabashi, 2004. Effects of a twin strain of Saccharomyces cerevisiae live cells on mixed ruminal microorganism fermentation in vitro. J. Anim. Sci., 82: 1847-1854.
PubMedDirect Link - Menke, K.H. and H. Steingass, 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev., 28: 7-55.
Direct Link - Minson, D.J. and R. Milford, 1966. The energy values and nutritive value indices of Digitaria decumbens, Sorghum almum and Phaseolus atropurpureus. Aust. J. Agric. Res., 17: 411-423.
CrossRefDirect Link - Mwenya, B., B. Santoso, C. Sar, Y. Gamo, T. Kobayashi, I. Arai and J. Takahashi, 2004. Effects of including β1-4 galacto-oligosaccharides, lactic acid bacteria or yeast culture on methanogenesis as well as energy and nitrogen metabolism in sheep. Anim. Feed Sci. Technol., 115: 313-326.
CrossRefDirect Link - Russell, J.B., J.D. O'Connor, D.G. Fox, P.J. Van Soest and C.J. Sniffen, 1992. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. J. Anim. Sci., 70: 3551-3561.
CrossRefPubMedDirect Link - Santoso, B., A. Maunatin, B.T. Hariadi and H. Abubakar, 2013. Isolation and identification of lactid acid bacteria originated from king grass (Pennisetum purpureophoides) as candidate of probiotic for livestock. J. Ilmu Ternak Veteriner, 18: 131-137.
CrossRefDirect Link - Santoso, B., E.W. Saragih and B.T. Hariadi, 2013. Effect of water extract of plants containing tannin on in vitro methagonesis and fermentation characteristics of the grass Pennisetum purpureophoides. J. Indonesian Trop. Anim. Agric., 38: 47-54.
CrossRefDirect Link - Santoso, B., B.T. Hariadi, Alimuddin and D.Y. Seseray, 2012. Fermentation characteristics of rice crop residue-based silage treated by epiphytic and commercial LAB. Media Peternakan-J. Anim. Sci. Technol., 35: 60-66.
Direct Link - Santoso, B., B.T. Hariadi and H. Abubakar, 2014. The effect of concentrate containing probiotics on fermentation characteristics, methanogenesis and in vitro nutrient digestility. J. Indonesian Trop. Anim. Agric., 39: 210-216.
CrossRefDirect Link - Seo, J.K., S.W. Kim, M.H. Kim, S.D. Upadhaya, D.K. Kam and J.K. Ha, 2010. Direct-fed microbials for ruminant animals. Asian-Aust. J. Anim. Sci., 23: 1657-1667.
CrossRefDirect Link - Tilley, J.M.A. and R.A. Terry, 1963. A two-stage technique for the in vitro digestion of forage crops. Grass Forage Sci., 18: 104-111.
CrossRefDirect Link - van Soest, P.J., 1994. Nutritional Ecology of the Ruminant. 2nd Edn., Cornell University Press, Ithaca, New York, Pages: 476.
Direct Link - van Soest, P.J., J.B. Robertson and B.A. Lewis, 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3597.
CrossRefPubMedDirect Link