Agbaje Rachael Bukola
Department of Food Science and Technology, Rufus Giwa Polytechnic, Owo, Nigeria
Oyetayo Victor Olusegun
Department of Microbiology, Federal University of Technology, P.M.B 704, Akure, Nigeria
Ojokoh Anthony Okhonloye
Department of Microbiology, Federal University of Technology, P.M.B 704, Akure, Nigeria
ABSTRACT
This research investigate the microbial, physical and proximate composition of tiger nut subjected to different fermentation methods such as traditional, back slope and control. Cleaned tiger nut were fermented for the period of four days in which samples were taken daily from each of the fermented groups to determine the total viable count of microorganisms associated with raw and fermented tiger nut samples, the pH and total titratable acidity. The result of total viable count reveal that back slope fermented milled sample has the highest microbial count of 4.16x105 cfu/g while control fermented sample show the least value (2.30x105 cfu/g). All the fermented samples show increment in lactic acid bacterial count throughout fermentation period. Traditional fermented whole sample had the highest fungal count of 4.40x105 sfu/g while control fermented sample showed the least value (9.33x104 sfu/g). The overall microorganisms isolated and identified from raw and fermented tiger nut were Streptococcus lactis, Lactobacillus plantarum, Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, Bacillus cereus, Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus and Penicillum italicum. There was significant decrease in pH value while total titratable acidity (TTA) increased in the fermented samples. Fermented whole sample had the highest protein value of 7.65% while raw sample had the least (4.2%) back slope fermented sample had the lowest moisture content (3.5%). There was no significant difference in the value of fat in all the samples. Back slope fermented sample had the highest value in ash (2.5%) and carbohydrate (65.0%) while the least value was recorded in the control fermented sample ash (0.5%) and carbohydrate (45.0%). Traditional and back slope fermentation was the best methods that may increase the proximate composition of tiger nut.
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How to cite this article
Agbaje Rachael Bukola, Oyetayo Victor Olusegun and Ojokoh Anthony Okhonloye, 2015. Assessment of the Microbial and Physico-Chemical Composition of Tigernut Subjected to Different Fermentation Methods. Pakistan Journal of Nutrition, 14: 742-748.
DOI: 10.3923/pjn.2015.742.748
URL: https://scialert.net/abstract/?doi=pjn.2015.742.748
DOI: 10.3923/pjn.2015.742.748
URL: https://scialert.net/abstract/?doi=pjn.2015.742.748
REFERENCES
- Adejuyitan, J.A., E.T. Otunola, E.A. Akande, I.F. Bolarinwa and F.M. Oladokun, 2009. Some physicochemical properties of flour obtained from fermentation of tigernut (Cyperus esculentus) sourced from a market in Ogbomoso, Nigeria. Afr. J. Food Sci., 3: 51-55.
CrossRefDirect Link - Ajayi, O.B. and F.L. Oyetayo, 2009. Potentials of Kerstingiella geocarpa as a health food. J. Med. Food, 12: 184-187.
CrossRefDirect Link - Belewu, M.A. and K.Y. Belewu, 2007. Comparative physico-chemical evaluation of tiger-nut, soybean and coconut milk sources. Int. J. Agric. Biol., 9: 785-787.
Direct Link - Borges, O., B. Goncalves, J.L.S. Carvalho, P. Correia and A.P. Silva, 2008. Nutritional quality of chestnut (Castanea sativa Mill.) cultivars from Portugal. Food Chem., 106: 976-984.
CrossRef - Chukwu, O.O.C., A.A. Chukwuedo, P. Otalike, I.D. Chukwu, N.O.G. Echeonwu, J.G. Bitrus and S.I. Akubo, 2013. Studies on food-borne bacteria in commercially hawked ready-to-eat fish in Jos and its environments. Afr. J. Food Sci., 7: 71-75.
Direct Link - Chukwuma, E.R., N. Obioma and O.I. Christopher, 2010. The phytochemical composition and some biochemical effects of Nigerian tigernut (Cyperus esculentus L.) tuber. Pak. J. Nutr., 9: 709-715.
CrossRefDirect Link - Coskuner, Y., R. Ercan, E. Karababa and A.N. Nazlican, 2002. Physical and chemical properties of chufa (Cyperus esculentus L) tubers grown in the Cukurova region of Turkey. J. Sci. Food Agric., 82: 625-631.
CrossRefDirect Link - Ihekoronye, A.I. and P.O. Ngoddy, 1985. Integrated Food Science and Technology for the Tropics. Macmillian Publisher, London, United Kingdom, ISBN: 9780333388839, Pages: 386.
Direct Link - Mohammed, S.I., L.R. Steenson and A.W. Kirleis, 1991. Isolation and characterization of microorganisms associated with the traditional sorghum fermentation for production of Sudanese kisra. Applied Environ. Microbiol., 57: 2529-2533.
Direct Link - Obizoba, I.C. and J.V. Atii, 1991. Effect of soaking, sprouting, fermentation and cooking on nutrient composition and some anti-nutritional factors of sorghum (Guinesia) seeds. Plant Foods Hum. Nutr., 41: 203-212.
CrossRefDirect Link - Oderinde, R.A. and O.A. Tairu, 1988. Evaluation of the properties of yellow nutsedge (Cyperus esculentus) tuber oil. Food Chem., 28: 233-237.
CrossRefDirect Link - Okafor, J.N.C., J.I. Mordi, A.U. Ozumba, H.M. Solomon and O. Olatunji, 2003. Preliminary studies on the characterization of contaminants in tigernut (Yellow variety). Proceedings of the 27th Annual Nigerian Institute of Food Science and Technology Conference, October 13-17, 2003, Kano, Nigeria, pp: 210-211.
- Oladele, A.K. and J.O. Aina, 2007. Chemical composition and functional properties of flour produced from two varieties of tiger nut (Cyperus esculentus). Afr. J. Biotechnol., 6: 2473-2476.
CrossRefDirect Link - Pascual, B., J.V. Maroto, S. Lopez-Galarza, A. Sanbautista and J. Alagarda, 2000. Chufa (Cyperus esculentus L. Var. Sativus boeck.): An unconventional crop. Studies related to applications and cultivation. Econ. Bot., 54: 439-448.
CrossRefDirect Link - Sanful, R.E., 2009. The use of Tiger-nut (Cyperus esculentus), cow milk and their composite as substrates for yoghurt production. Pak. J. Nutr., 8: 755-758.
CrossRefDirect Link - Rubert, J., N. Sebastia, J.M. Soriano, C. Soler and J. Manes, 2011. One-year monitoring of aflatoxins and ochratoxin a in tiger-nuts and their beverages. Food Chem., 127: 822-826.
CrossRefDirect Link - Wakil, S.M., O.T. Ayenuro and K.A. Oyinlola, 2014. Microbiological and nutritional assessment of starter-developed fermented tigernut milk. Food Nutr. Sci., 5: 495-506.
CrossRefDirect Link