Maria Carolina Goncalves Pita
Departamento de Clinica Medica, da Faculdade de Medicina Veterinaria e Zootecnia, da Universidade de Sao Paulo, Orlando Marques de Paiva, no 87, CEP 05508-270, Sao Paulo, SP, Brazil
Paulo Reis de Carvalho
Departamento de Clinica Medica, da Faculdade de Medicina Veterinaria e Zootecnia, da Universidade de Sao Paulo, Orlando Marques de Paiva, no 87, CEP 05508-270, Sao Paulo, SP, Brazil
Eduardo Piber Neto
Departamento de Clinica Medica, da Faculdade de Medicina Veterinaria e Zootecnia, da Universidade de Sao Paulo, Orlando Marques de Paiva, no 87, CEP 05508-270, Sao Paulo, SP, Brazil
Cassio Xavier de Mendonca
Departamento de Clinica Medica, da Faculdade de Medicina Veterinaria e Zootecnia, da Universidade de Sao Paulo, Orlando Marques de Paiva, no 87, CEP 05508-270, Sao Paulo, SP, Brazil
ABSTRACT
Hundred forty-four Shaver White laying hens were used over a 4 week experimental period to investigate the effect of 3% of soybean oil, corn oil (MIL), canola oil, flaxseed oil (LIN), salmon oil (SAL) or tuna and sardine oil (SR/AT) added to the diets, upon the fatty acid egg yolk composition, blood plasma levels and incorporation time of each fatty acid into the egg yolk. Hens were allocated into 72 cages and the experimental design was a 6 x 6 randomized factorial model. Hens fed 3% of different oils, responded with increased polyunsaturated fatty acids omega 3 (ω-3 PUFAs), except for corn oil. The addition of flaxseed, soybean or corn oil into the diet increased the PUFAs levels into the egg yolk and in the blood plasma. Adding tuna and sardine oil into the diet increased the concentration of yolk saturated fatty acids. The levels of ω-3 PUFAs were increased in the tuna and sardine oil treatment, while the flaxseed oil increased the plasma fatty acids. The deposition of 349.28 mg/yolk of a-linolenic fatty acids (ALA) was higher in the group fed LIN, while the higher equal to 157.13 mg DHA/yolk was observed in group SR/AT. In the plasma, deposition increased from 0.33% (MIL) for 6.29% ALA (LIN), while that of DHA increase of 0.47% (MIL) for 4.24% (SAL) and 4.48% (SR/AT) and of 0.98% (MIL) for 6.14% (SR/AT) and 8.44% (LIN) of ω-3 PUFAs. The percentage of EPA into the yolk and plasma was higher for the hens fed 3% tuna and sardine oil diet, as well as the levels of yolk DHA. The concentration of DHA into the plasma was higher for the salmon and tuna/sardine oil treatments. The PUFAs yolk decreased during the first eight days of experiment, while the ω-3 PUFAs increased during the same period. The concentration of ALA increased until ten days of experiment, while the percentage of EPA and DHA increased up to the eighth experimental day.
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How to cite this article
Maria Carolina Goncalves Pita, Paulo Reis de Carvalho, Eduardo Piber Neto and Cassio Xavier de Mendonca, 2011. Modulation of Plasma Levels and Percentages of Incorporation of ω-3 PUFAs in Egg Yolk under the Influence of Supplementation Sources Rich in Omega 3 to Diet of Laying Hens. Pakistan Journal of Nutrition, 10: 735-754.
DOI: 10.3923/pjn.2011.735.754
URL: https://scialert.net/abstract/?doi=pjn.2011.735.754
DOI: 10.3923/pjn.2011.735.754
URL: https://scialert.net/abstract/?doi=pjn.2011.735.754
REFERENCES
- Ahn, D.U., H.H. Sunwoo, F.H. Wolfe and J.S. Sim, 1995. Effects of dietary α-linolenic acid and strais of hen on the fatty acid composition, storage stability and flavor characteristics of chicken eggs. Poult. Sci., 74: 1540-1547.
PubMed - Aymond, W.M. and M.E. van Elswyk, 1995. Yolk thiobarbituric acid reactive substances and n-3 fatty acids in response to whole and ground flaxeed. Poult. Sci., 74: 1388-1394.
PubMedDirect Link - Bang, H.O. and J. Dyerberg, 1972. Plasma lipids and lipoproteins in Greenlandic west cost Eskimos. Acta Med. Scand., 192: 85-94.
CrossRefDirect Link - Baucells, M.D., N. Crespo, A.C. Barroeta, S. Lopez-Ferrer and M.A. Grashorn, 2000. Incorporation of different polyunsaturated fatty acids into eggs. Poult. Sci., 79: 51-59.
PubMedDirect Link - Bickerstaffe, R. and E.F. Annison, 1970. Lipid metabolism in the perfused chicken liver. The uptake and metabolism of oleic acid, elaidic acid, cis-vaccenic acid, trans-vaccenic acid and stearic acid. Biochem. J., 118: 433-442.
PubMed - Bligh, E.G. and W.J. Dyer, 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37: 911-917.
CrossRefPubMedDirect Link - Cachaldora, P., P. Garcoia-Rebollar, C. Alvarez, J.C. De Blas and J. Mendez, 2006. Effect of type and level of fish oil supplementation on yolk fat composition and n-3 fatty acids retention efficiency in laying hens. Br. Poult. Sci., 47: 43-49.
PubMed - De Carvalho, P.R., M.C.G. Pita, E.P. Neto and C.X. De Mendonca, 2009. Efficiency of PUFAs incorporation from marine sources in yolk egg's laying hens. Int. J. Poult. Sci., 8: 603-614.
CrossRefDirect Link - Chen, P.H., R.H. Common, N. Nikolaiczuk and H.F. Macrae, 1965. Some effects of added dietary fats on the lipid composition of hen's egg yolk. J. Food Sci., 30: 838-845.
CrossRefDirect Link - Cherian, G. and J.S. Sim, 2001. Maternal dietary alpha-linolenic acid (18:3n-3) alters n-3 polyunsaturated fatty acid metabolism and liver enzyme activity in hatched chicks. Poult. Sci., 80: 901-905.
Direct Link - Cherian, G., F.W. Wolfe and J.S. Sim, 1996. Feeding dietary oils with tocopherols: Effects on internal qualities of eggs during storage. J. Food Sci., 61: 15-18.
CrossRef - Cherian, G., F.W. Wolfe and J.S. Sim, 1996. Dietary oils with added tocopherols: effects on egg or tissue tocopherols, fatty acids, and oxidative stability. Poult. Sci., 75: 423-431.
CrossRefPubMedDirect Link - Cherian, G. and J.S. Sim, 1992. Ω-3 fatty acid and cholesterol content of newly hatched chick from α-linolenic acid enriched eggs. Lipids, 27: 706-710.
CrossRefPubMedDirect Link - Connor, W.E., M. Neuringer and S. Reisbick, 1991. Essentiality of omega 3 fatty acids: Evidence from the primate model and implications for human nutrition. World Rev. Nutr. Diet., 66: 118-132.
PubMed - Dangour, A.D., E. Allen, D. Elbourne, N. Fasey and A.E. Fletcher et al., 2010. Effect of 2-y n-3 long-chain polyunsaturated fatty acid supplementation on cognitive function in older people: A randomized, double-blind, controlled trial. Am. J. Clin. Nutr., 91: 1725-1732.
Direct Link - Folch, J., M. Lees and G.H.S. Stanley, 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226: 497-509.
CrossRefPubMedDirect Link - Galobart, J., A.C. Barroeta, M.D. Baucells, L. Cortinas and F. Guardiola, 2001. α-Tocopherol transfer efficiency and lipid oxidation in fresh and spray-dried eggs enriched with ω3-polyunsaturated fatty acids. Poult. Sci., 80: 1496-1505.
PubMed - Galobart, J., A.C. Barroeta, M.D. Baucells and F. Guardiola, 2001. Lipid oxidation in fresh and spray-dried eggs enriched with omega3 and omega6 polyunsaturated fatty acids during storage as affected by dietary vitamin E and canthaxanthin supplementation. Poult. Sci., 80: 327-337.
Direct Link - Grobas, S., J. Mendez, R. Lazaro, C. de Blas and G.G. Mateo, 2001. Influence of source and percentage of fat added to diet on performance and fatty acid composition of egg yolks of two strains of laying hens. Poult. Sci., 80: 1171-1179.
CrossRefDirect Link - Hamilton, R.M.G., 1982. Methods and factors that affect the measurement of egg shell quality. Poult. Sci., 61: 2022-2039.
Direct Link - Hargis, P.S., 1988. Modifying egg yolk cholesterol in the domestic fowl-A review. World's Poult. Sci. J., 44: 17-29.
CrossRefDirect Link - Hargis, P.S., M.E. van Elswyk and B.M. Hargis, 1991. Dietary modification of yolk lipid with menhaden oil. Poult. Sci., 70: 874-883.
PubMed - Hargis, P.S. and M.E. van Elswyk, 1993. Manipulating the fatty acid composition of poultry meat and eggs for the health conscious consumer. World's Poult. Sci. J., 49: 251-264.
CrossRefDirect Link - Hartman, L. and R.C.A. Lago, 1973. Rapid preparation of fatty acid methyl esters from lipids. Lab. Pract., 22: 475-476.
PubMedDirect Link - Heald, P.J. and H.G. Badman, 1963. Lipid metabolism and the laying hen. I. Plasma-free fatty acids and the onset of laying in the domestic fowl. Biochim. Biophys. Acta., 70: 381-388.
CrossRef - Herber, S.M. and M.E. van Elswyk, 1996. Dietary marine algae promotes efficient deposition of n-3 fatty acids for the production of enriched shell eggs. Poult. Sci., 75: 1501-1507.
PubMedDirect Link - Herod, P.M. and J.E. Kinsella, 1986. Fish oil consumption and decreased risk of cardiovascular disease: A comparison of findings from animal and human feeding trials. Am. J. Clin. Nutr., 43: 566-598.
PubMedDirect Link - Ivy, C.A. and M.C. Nesheim, 1973. Factors influencing the liver fat content of laying hens. Poult. Sci., 52: 281-291.
Direct Link - Kinsella, J.E., B. Lokesh and R.A. Stone, 1990. Dietary n-3 polyunsaturated fatty acid and amelioration of cardiovascular disease: Possible mechanisms. Am. J. Clin. Nutr., 52: 1-28.
PubMed - Kudzma, D.J., St.C. Felix, L. DeLallo and S.J. Friedberg, 1975. Mechanism of avian estrogen-induced hypertriglyceridemia: Evidence for overproduction of triglyceride. J. Lipid Res., 16: 123-133.
Direct Link - Kudzma, D.J., J.B. Swaney and E.N. Ellis, 1979. Effects of estrogen administration on the lipoproteins and apoproteins of the chicken. Biochim. Biophys. Acta., 572: 257-268.
CrossRef - Leskanich, C.O. and R.C. Noble, 1997. Manipulation of the n-3 polyunsaturated fatty acid composition of avian eggs and meat. World's Poult. Sci. J., 53: 155-183.
CrossRefDirect Link - Leveille, G.A., D.R. Romsos, Y. Yeh and E.K. O'Hea, 1975. Lipid biosynthesis in the chick. A consideration of site of synthesis, influence of diet and possible regulatory mechanisms. Poult. Sci., 54: 1075-1093.
CrossRefPubMedDirect Link - Lewis, N.M., S. Seburg and N.L. Flanagan, 2000. Enriched eggs as a source of N-3 polyunsaturated fatty acids for humans. Poult. Sci., 79: 971-974.
CrossRefDirect Link - Liu, C.C.F., S.E. Carlson, P.G. Rhodes, U.S. Rao and E.F. Meydrech, 1987. Increase in plasma phospholipid docosahexanoic and eicosa-pentaenoic acids as a reflection of their intake and mode of administration. Pediatry Res., 22: 292-296.
PubMed - Lopez-Ferrer, S., M.D. Baucells, A.C. Barroeta and M.A. Grashorn, 1999. N-3 enrichment of chicken meat using fish oil: Alternative substitution with rapeseed and linseed oils. Poult. Sci., 78: 356-365.
CrossRefPubMedDirect Link - Marshall, A.C., K.S. Kubena, K.R. Hinton, P.S. Hargis and M.E. Van Elswyk, 1994. n-3 fatty acid enriched table eggs: A survey of consumer acceptability. Poult. Sci., 73: 1334-1340.
PubMed - Moran Jr. E.T., 1996. Fat modification of animal products for human consumption. Anim. Feed Sci., Techn., 58: 91-99.
Direct Link - Naber, E.C. and M.D. Biggert, 1989. Patterns of lipogenesis in laying hens fed a high fat diet containing safflower oil. J. Nutr., 119: 690-695.
Direct Link - Nash, D.M., R.M.G. Hamilton and H.W. Hulan, 1995. The effect of dietary herring meal on the ω-3 fatty acid content of plasma and egg yolk lipids of laying hens. Can. J. Anim. Sci., 75: 247-253.
CrossRefDirect Link - National Rearch Council NRC, 1989. Recommended Dietary Allowances. 10th Edn., National Academy Press, Washington, DC. USA., pp: 284.
Direct Link - Nelson, G.J. and R.G. Ackman, 1988. Absorption and transport of fat in mammals with emphasis on n-3 polyunsaturated fatty acids. Lipids, 23: 1005-1014.
PubMed - Nettleton, J.A., 1991. Omega-3 fatty acids: Comparison of plant and seafood sources in human nutrition. J. Am. Diet. Assoc., 91: 331-337.
PubMedDirect Link - Neuringer, M., G.J. Anderson and W.E. Conner, 1998. The essentiality of ω-3 fatty acids for the development and function of the retina and brain. Ann. Rev. Nutr., 8: 517-541.
CrossRef - Neuringer, M., 2000. Infant vision and retinal function in studies of dietary long-chain polyunsaturated fatty acids: Methods, results and implications. Am. J. Clin. Nutr., 71: 256S-267S.
Direct Link - Nielsen, H., 1998. Hen age and fatty acid composition of egg yolk lipid. Br. Poult. Sci., 39: 53-56.
CrossRefDirect Link - Noble, R.C. and M. Cocchi, 1990. Lipid metabolism and the neonatal chicken. Progr. Lipid Res., 29: 107-140.
CrossRefPubMedDirect Link - Noble, R.C. and M. Cocchi, 1990. Lipid metabolism and the neonatal chicken. Progr. Lipid Res., 29: 107-140.
CrossRefPubMedDirect Link - Noble, R.C., F. Lonsdale, K. Connor and D. Brown, 1986. Changes in the lipid metabolism of the chick embryo with parental age. Poult. Sci., 65: 409-416.
PubMed - Noble R.C., B.K. Speake, R.J. McCartney, C.M. Foggin and D.C. Deeming, 1996. Yolk lipids and their fatty acids in the wild and captive ostrich (Struthio camelus). Comp. Biochem. Physiol., 113: 753-756.
CrossRef - Scheideler, S.E. and G.W. Froning, 1996. The combined influence of dietary flaxseed variety, level, form and storage conditions on egg production and composition among vitamin-E supplemented hens. Poult. Sci., 75: 1221-1226.
Direct Link - Schreiner, M., H.W. Hulan, E. Razzazi-Fazeli, J. Bohm and C. Iben, 2004. Feeding laying hens seal blubber oil: Effects on egg yolk incorporation, stereospecific distribution of omega-3 fatty acids, and sensory aspects. Poult. Sci., 83: 462-473.
Direct Link - Shapiro, J.A., T.D. Koepsell, L.F. Voigt, C.E. Dugowson, M. Kestin and J.L. Nelson, 1996. Diet and rheumatoid artritis in women: A possible protective effect of fish consumption. Epidemiol., 7: 256-263.
PubMed - Sim, J.S. and D.B. Bragg, 1977. Effect of dietary factors on serum and egg yolk cholesterol levels of laying hens. Poult. Sci., 56: 1616-1621.
PubMed - Simopoulos, A.P., 2000. Human requirement of n-3 polyunsaturated fatty acids. Poult. Sci., 79: 961-970.
PubMed - Simopoulos, A.P., 2009. Evolutionary aspects of the dietary omega-6:omega-3 fatty acid ratio: Medical implications. World Rev. Nutr. Diet., 100: 1-21.
PubMed - Teitelbaum, J.E. and W.A. Walker, 2001. Review: The role of omega 3 fatty acids in intestinal inflammation. J. Nutr. Biochem., 12: 21-32.
PubMed - Uauy, R., P. Peirano, D. Hoffman, P. Mena, D. Birch and E. Birch, 1996. Role of essential fatty acids in the function of the developing nervous system. Lipids, 31: 167-176.
PubMed - Van Elswyk, M.E., B. Hargis, J. Williams and P. Hargis, 1994. Dietary menhaden oil contributes to hepatic lipidosis in laying hens. Poult. Sci., 73: 653-662.
PubMedDirect Link