S. Janakat
Department of Nutrition and Food Technology, Jordan University of Science and Technology, P.O. Box 3030, Irbid, Jordan
M. Nassar
Department of Nutrition and Food Technology, Jordan University of Science and Technology, P.O. Box 3030, Irbid, Jordan
ABSTRACT
Hepatoprotective activity of Terfezia claveryi aqueous, methanolic and petroleum ether extracts was evaluated in the rat using a potent hepatotoxin carbon tetrachloride (CCl4) in comparison with the hepatoprotective activity of a reference plant Nigella sativa. The extracts were administrated via gavage three days prior to CCl4 intoxication followed by two additional doses one hour and four hours after CCl4 injection. Twenty four hours after intoxication, blood samples were collected and serum bilirubin concentration, Alkaline Phosphatase (ALP), Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) activities were measured. Body weight was measured then livers were excises and livers were weighed. The aqueous, methanolic and petroleum ether extracts of T. claveryi and N. sativa lowered all liver function tests significantly. However, the aqueous extract of T. claveryi almost normalized the effect of CCl4 and was as effective as the petroleum ether extract of the reference plant N. sativa. Moreover, the aqueous extract of T. claveryi normalized CCl4 induced hepatomegaly, which was comparable to the effect of petroleum ether extract of N. sativa. These results demonstrate that aqueous extract of T. claveryi possesses a very powerful hepatoprotective activity against CCl4 and it is as effective as petroleum ether extract of the reference plant N. sativa.
PDF References
How to cite this article
S. Janakat and M. Nassar, 2010. Hepatoprotective Activity of Desert Truffle (Terfezia claveryi) in Comparison with the Effect of Nigella sativa in the Rat. Pakistan Journal of Nutrition, 9: 52-56.
DOI: 10.3923/pjn.2010.52.56
URL: https://scialert.net/abstract/?doi=pjn.2010.52.56
DOI: 10.3923/pjn.2010.52.56
URL: https://scialert.net/abstract/?doi=pjn.2010.52.56
REFERENCES
- Bokhary, H.A. and S. Parvez, 1993. Chemical composition of desert truffles Terfezia claveryi. J. Food Comp. Anal., 6: 285-293.
CrossRef - Burits, M. and F. Bucar, 2000. Antioxidant activity of Nigella sativa essential oil. Phytother. Res., 14: 323-328.
CrossRefPubMedDirect Link - Daba, M.H. and M.S. Abdel-Rahman, 1998. Hepatoprotective activity of thymoquinone in isolated rat hepatocytes. Toxicol. Lett., 95: 23-29.
CrossRefDirect Link - El-Dakhakhny, M., N.I. Mady and M.A. Halim, 2000. Nigella sativa L. oil protects against induced hepatotoxicity and improves serum lipid profile in rats. Arzneimittelforschung, 50: 832-836.
CrossRefPubMedDirect Link - El Daly, E.S., 1998. Protective effect of cysteine and vitamin E, Crocus sativus and Nigella sativa extracts on cisplatin-induced toxicity in rats. J. Pharmacie Belgique, 53: 87-93.
PubMedDirect Link - El Tahir, K.E.H., M.M.S. Ashour and M.M. Al-Harbi, 1993. The respiratory effects of the volatile oil of the black seed (Nigella sativa) in guinea-pigs: Elucidation of the mechanism(s) of action. Gen. Pharmacol.: Vasc. Syst., 24: 1115-1122.
CrossRefPubMedDirect Link - Gazzani, G., A. Papetti, M. Daglia, F. Berte and C. Gregotti, 1998. Protective activity of water soluble components of some common diet vegetables on rat liver microsome and the effect of thermal treatment. J. Agric. Food Chem., 46: 4123-4127.
Direct Link - Gazzani, G., A. Papetti, G. Massolini and M. Daglia, 1998. Anti- and prooxidant activity of water soluble components of some common diet vegetables and the effect of thermal treatment. J. Agric. Food Chem., 46: 4118-4122.
CrossRefDirect Link - Houghton, P.J., R. Zarka, B. de las Heras and J.R.S. Hoult, 1995. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med., 61: 33-36.
CrossRefPubMedDirect Link - Huang, W., J. Zhang, M. Washington, J. Liu, J.M. Parant, G. Lozano and D.D. Moore, 2005. Xenobiotic stress induces hepatomegaly and liver tumors via the nuclear receptor constitutive androstane receptor. Mol. Endocrinol., 19: 1646-1653.
CrossRefPubMedDirect Link - Janakat, S. and H. Al-Merie, 2002. Optimization of the dose and route of injection and characterization of the time course of carbon tetrachloride-induced hepatotoxicity in the rat. J. Pharmacol. Toxicol. Methods, 48: 41-44.
CrossRef - Janakat, S. and H. Al-Merie, 2002. Evaluation of hepatoprotective effect of Pistacia lentiscus, Phillyrea latifolia and Nicotiana glauca. J. Ethnopharmacol., 83: 135-138.
CrossRefDirect Link - Kanter, M., O. Coskun and M. Budancamanak, 2005. Hepatoprotective effects of Nigella sativa L. and Urtica dioica L. on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. World J. Gastroenterol., 42: 6684-6688.
PubMedDirect Link - Mehendale, H.M., R.A. Roth, A.J. Gandolfi, J.E. Klaunig, J.J. Lemasters and L.R. Curtis, 1994. Novel mechanism in chemically induced hepatotoxicity. FASEB J., 8: 1285-1295.
Direct Link - Muchizuki, M., S. Shimizu, Y. Urasoko, K. Umisheta, T. Kamata and T. Kitazawa et al., 2009. Carbon tetrachloride hepatotoxicity in pregnant and lactating rats. Toxicol. Sci., 34: 175-181.
PubMedDirect Link - Antonia, M., M. Martinez-Tome, A.M. Jimenez, M. Vera Ana, M. Honrubia and P. Parras, 2002. Antioxidant activity of edible fungi (truffles and mushroom): Losses during industrial processing. J. Food Prot., 65: 1614-1622.
Direct Link - Nielsen, A.V., T.E. Christensen, M. Bojk and J. Marcussen, 1997. Purification and characterization of β-amylase from leaves of potato (Solanum tuberosum). Physiol. Plant., 99: 190-196.
CrossRef - Ramadan, M.F., L.W. Kroh and J.T. Morsel, 2003. Radical scavenging activity of black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.) and niger (Guizotia abyssinica cass.) crude seed oils and oil fractions. J. Agric. Food Chem., 51: 6961-6969.
CrossRefDirect Link - Reitman, S. and S. Frankel, 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28: 56-63.
CrossRefPubMedDirect Link - Recknagel, R.O., 1967. Carbon tetrachloride hepatotoxicity. Pharmacol. Rev., 19: 145-208.
Direct Link - Recknagel, R.O., E.A. Glende, Jr., J.A. Dolak and R.L. Waller, 1989. Mechanisms of carbon tetrachloride toxicity. Pharmacol. Ther., 43: 139-154.
CrossRefPubMedDirect Link - Turkdogan, M.K., Z. Agaoglu, Z. Yener, R. Sekeroglu, H.A. Akkan and M.E. Avci, 2001. The role of antioxidant vitamins (C and E), selenium and Nigella sativa in the prevention of liver fibrosis and cirrhosis in rabbits: New hopes. Dtsch. Tierarztl. Wochenschr., 108: 71-73.
PubMedDirect Link - Turkdogan, M.K., H. Ozbek, Z. Yener, I. Tuncer, I. Uygan and E. Ceylan, 2003. The role of Urtica dioica and Nigella sativa in the prevention of carbon tetrachloride-induced hepatotoxicity in rats. Phytother. Res., 17: 942-946.
PubMedDirect Link