Sanro Tachibana
Department of Applied Bioscience, Faculty of Agriculture,
Ehime University 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan
Yukinori Kiyota
Department of Applied Bioscience, Faculty of Agriculture,
Ehime University 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan
Michifusa Koga
Department of Applied Bioscience, Faculty of Agriculture,
Ehime University 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan
ABSTRACT
Microbial degradation of 2,7-Dichlorodibenzo-p-Dioxin (2,7-DCDD) and 2,4,8-Trichlorodibenzofuran (2,4,8-TCDF) with four fungi (PL1, PL2, PC and 267) screened from nature was conducted to select fungi for the bioremediation of dioxins in soil. The four fungi degraded 63 to 96% of 2,7-DCDD and 24 to 78% of 2,4,8-TCDF, respectively. Maximal degradation (96 and 78%) was obtained with PL1 when the incubation was conducted for 30 days after addition of 0.25 mM of 2,7-DCDD and 2,4,8-TCDF to the culture medium, respectively. Two fungi (PL1 and 267) were selected for bioremediation experiments. In 15 and 30 days, 267 degraded 37 to 63% of 2,7-DCDD and 18 to 58% of 2,4,8-TCDF, present at 1 and 10 ppm in soil. Maximal degradation (63 and 58%) was obtained with 267 when the bioremediation was conducted for 30 days after addition of 10 ppm of 2,7-DCDD and 2,4,8-TCDF to the soil, respectively. Furthermore, the degradation of 2,7-DCDD was enhanced using a solid medium. Also, 2,7-DCDD and 2,4,8-TCDF were indeed degraded by the fungi (267 and PL1), because 4-Chlorocatechol and 3,5-Dichlorosalicylic acid and 5-Chlorosalicylic acid, considered to be the intermediates in the bioremediation of 2,7-DCDD and 2,4,8-TCDF, respectively, were detected among the reaction products.
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How to cite this article
Sanro Tachibana, Yukinori Kiyota and Michifusa Koga, 2005. Biodegradation of 2,7-Dibenzo-p-Dixoin and 2,4,8-Trichlorodibenzofuran in Soil by Fungi Screened from Nature. Pakistan Journal of Biological Sciences, 8: 1751-1757.
DOI: 10.3923/pjbs.2005.1751.1757
URL: https://scialert.net/abstract/?doi=pjbs.2005.1751.1757
DOI: 10.3923/pjbs.2005.1751.1757
URL: https://scialert.net/abstract/?doi=pjbs.2005.1751.1757
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