M. A. Kashem
Department of Botany, University of Chittagong, Chittagong-4331, Bangladesh
M. A. Manchur
Department of Microbiology,
M. S. Rahman
Department of Microbiology,
M. N. Anwar
Department of Microbiology,
ABSTRACT
Two thermophilic cellulolytic bacterial isolates were tested to determine the effect of carbon and nitrogen sources on the production of extra-cellular proteins, reducing sugars and cellulolytic enzymes. Lactose was found to be the most potential carbon source for Avicelase (342.52 U mL-1) and ß-glucosidase (256.89 U mL-1) activity where as NH4Cl was found to be the potential nitrogen source for CMCase (144.68 U mL-1) activity.
PDF References Citation
How to cite this article
M. A. Kashem, M. A. Manchur, M. S. Rahman and M. N. Anwar, 2004. Effect of Carbon and Nitrogen Sources on the Production of Reducing Sugars,
Extra-cellular Protein and Cellulolytic Enzymes by Two Cellulolytic Bacterial
Isolates. Pakistan Journal of Biological Sciences, 7: 1660-1663.
DOI: 10.3923/pjbs.2004.1660.1663
URL: https://scialert.net/abstract/?doi=pjbs.2004.1660.1663
DOI: 10.3923/pjbs.2004.1660.1663
URL: https://scialert.net/abstract/?doi=pjbs.2004.1660.1663
REFERENCES
- Stutzenberger, F.J., 1971. Cellulase production by Thermomonospora curvata isolated from municipal solid waste compost. J. Applied Microbiol., 22: 147-152.
PubMed - Buchanan, R.E. and N.E. Gibbons, 1974. Bergey's Manual of Determinative Bacteriology. 8th Edn., Williams and Wilkins Co., Baltimore, MD., pp: 747-842.
CrossRefDirect Link - Nelson, N., 1944. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem., 153: 375-380.
CrossRefDirect Link - Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
CrossRefPubMedDirect Link - Henriette, C., S. Zinebi, M.F. Aumaitre, E. Petitdemange and H. Petitdemange, 1993. Protease and lipase production by a strain of Serratia marcescens. J. Ind. Microbiol., 12: 129-135.
CrossRefDirect Link - Mandels, M. and E.T. Reese, 1957. Induction of cellulase in Trichderma viride as infuenced by carbon sources and materials. J. Bacteriol., 73: 269-278.
Direct Link