Zaied K. A.
Department of Genetics, Faculty of Agriculture, Mansour a University, Egypt
A.H. Abd El-Hady
Department of Genetics, Faculty of Agriculture, Mansour a University, Egypt
ABSTRACT
The potency of two Bacillus strains and four new isolates of transconjugants was determined against Spodoptera littoralis. Most of bacterial transconjugants revealed insignificant correlation between the average consumption of fed leaves with the time of treatment during the age of larvae if a mixture of relatively pure crystals and endospores was used, similar to that resulting from either fraction of crystals protein alone. This suggested that feeding was reduced by the B. thuringiensis products. The bacterial preparation containing crystals and endospores from transconjugant-D revealed high levels of toxicity for the negative correlation obtained between the average consumption of fed leaves with the larval age. Highly significant correlation was obtained between mortality and the larval age using bacterial preparations containing crystals alone from trans-conjugants A and D, as well as, that containing crystals and endospores from all bacterial strains and their transconjugants. In most cases, transconjugant preparations causing high levels of mortality than parental strains, because of high toxicity against S. littoralis larvae. The new product of transconjugant-B containing crystals and endospores appeared to have insignificant correlation between treatment time and the average weight of surviving larvae. It was indicated that this product increased pathogenicity and decreased both the growth and development of S. littoralis larvae. This new product from transconjugant-B decreased the average consumption of fed leaves and the average weight of surviving larvae and it would be preferable against S. littoralis in biological control. The results showed that higher levels of mortality, if a mixture of relatively pure crystals and spores was used as compared to either fraction of crystals protein alone.
PDF References Citation
How to cite this article
Zaied K. A. and A.H. Abd El-Hady, 2001. Potency and Toxicity of Bacterial Preparations from Trans-conjugants of Bacillus thuringiensis Against Larvae of Spodoptera littoralis. Pakistan Journal of Biological Sciences, 4: 722-727.
DOI: 10.3923/pjbs.2001.722.727
URL: https://scialert.net/abstract/?doi=pjbs.2001.722.727
DOI: 10.3923/pjbs.2001.722.727
URL: https://scialert.net/abstract/?doi=pjbs.2001.722.727
REFERENCES
- Adams, L., K. Brown and H. Whiteley, 1992. Molecular cloning and characterization of two genes encoding sigma factors that direct transcription from a Bacillus thuringiensis crystal protein gene promoter. J. Bacteriol., 173: 3846-3854.
Direct Link - Dulmage, H.T., O.P. Boening, C.S. Rehnborg and G.D. Hansen, 1971. A proposed standardized bioassay for formulations of Bacillus thuringiensis based on the international unit. J. Invertebr. Pathol., 18: 240-245.
CrossRef - Hannay, C.L., 1953. Crystalline inclusions in aerobic sporeforming bacteria. Nature, 172: 1004-1004.
CrossRefDirect Link - Heimpel, A.M., 1967. A critical review of Bacillus thuringiensis var thuringiensis Berliner and other crystalliferous bacteria. Ann. Rev. Entomol., 12: 287-322.
CrossRef - Karamanlidou, G., A.F. Lambropoulos, S.I. Koliais, T. Manousis, D. Ellar and C. Kastritsis, 1991. Toxicity of Bacillus thuringiensis to laboratory populations of the olive fruit fly (Dacus oleae). Applied Environ. Microbiol., 57: 2277-2282.
Direct Link - Navon, A., M. Wysoki and S. Keren, 1983. Potency and effect of Bacillus thuringiensis preparations against larvae of Spodoptera littoralis and Boarmia (Ascotis) selenaria. Phytoparasitica, 11: 3-11.
CrossRef - Puntamabeker, U.S. and P.K. Ranjekor, 1989. Intergeneric protoplast fusion between Agrobacterium tumefaciens and Bacillus thuringiensis subsp. Kurstaki. Biotechnol. Lett., 11: 717-722.
CrossRef - Sneh, B., S. Schuster and M. Broza, 1981. Insecticidal activity of Bacillus thuringiensis strains against the Egyptian cotton leafworm, Spodoptera littoralis (Lep.: Noctuidae). Entomophaga, 26: 179-190.
CrossRefDirect Link - Steel, R.G.D. and J.H. Torrie 1960. Principles and Procedures of Statistics. McGraw Hill, New York, USA, ISBN: 9780070609259, Pages: 481.
Direct Link - Thomas, W.E. and D.J. Ellar, 1983. Bacillus thuringiensis var israelensis crystal ó-endotoxin: Effects of insect and mammalian cells in vitro and in vivo. J. Cell Sci., 60: 181-197.
Direct Link - Whiteley, H.R. and H.E. Schnepf, 1986. The molecular biology of parasporal crystal body dormation in Bacillus thuringiensis. Ann. Rev. Microbiol., 40: 549-576.
PubMed - Zaied, K.A., 2000. Relative susceptibility of Egyptian cotton leafworm to a new recombinant preparations of Bacillus thuringiensis. Egypt. J. Microbiol., 36: 313-328.
Direct Link - Zaied, K.A., 2000. Screening of the insecticidal activity of new recombinant preparations of Bacillus thuringiensis var. kurstaki against the Egyptian cotton leafworm. Pak. J. Biol. Sci., 3: 753-758.
CrossRefDirect Link