Fabiola de S.R.G. Garrido
Biochemistry, NCPS, IMS-CAT, UFBA, Viloria da Conquista-BA, Brazil
Rodrigo Grazinoli Garrido
Biochemistry, NCPS, IMS-CAT, UFBA, Viloria da Conquista-BA, Brazil
Carlos Alberto Bucher
Biochemistry, NCPS, IMS-CAT, UFBA, Viloria da Conquista-BA, Brazil
Sonia Regina de Souza
Biochemistry, NCPS, IMS-CAT, UFBA, Viloria da Conquista-BA, Brazil
Manlio Silvestre Fernandes
Biochemistry, NCPS, IMS-CAT, UFBA, Viloria da Conquista-BA, Brazil
ABSTRACT
In a growth chamber experiment, the response of two rice varieties (Piaui and IAC-47) to nitrate suppression (24 h) and pulses (72 h) was studied. Nitrate suppression in nutrient solution increased plasma membrane H+-ATPase activity in Piaui plants previously grown under 0.5 mM and IAC-47 under 5.0 mM NO3-N. Under NO3- pulses, IAC-47 presented highest H+-ATPase activity in its plasma membrane, while Piaui showed increases in tonoplast V-H+-ATPase activity. These results suggested that under nitrate pulses IAC-47 increased its ability to take up NO3- from nutrient solution. Piaui plants preferentially retrieves nitrate stored from the vacuoles. Plasmalem protein content in Piaui tonoplast was enhanced particularly in -N plants previously grown under 5.0 mM NO3--N. We suggest that these enzymes (H+-ATPases) could be monitored as markers in nitrogen use efficiency study in rice plants.
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How to cite this article
Fabiola de S.R.G. Garrido, Rodrigo Grazinoli Garrido, Carlos Alberto Bucher, Sonia Regina de Souza and Manlio Silvestre Fernandes, 2008. Rice Varieties Tonoplast and Plasma Membrane H+-ATPases Differential Activities in Response to Nitrate Pulses. Journal of Biological Sciences, 8: 107-112.
DOI: 10.3923/jbs.2008.107.112
URL: https://scialert.net/abstract/?doi=jbs.2008.107.112
DOI: 10.3923/jbs.2008.107.112
URL: https://scialert.net/abstract/?doi=jbs.2008.107.112
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