Yu. P. Petrov
Department of Cell Culture, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia
ABSTRACT
A computer model for transcription along the major groove of DNA without formation of the transcription bubble is presented. The model is based on the following assumptions. (1) Transcription should occur without alteration of the DNA B-form. (2) The DNA template strand and the transcribed RNA are antiparallel. (3) Specificity of the transcribed RNA is exclusively determined by hydrogen bonds; the number of the DNA-RNA hydrogen bonds is one per triplet. (4) The distance from the sugar-phosphate backbone to the DNA axis does not dependent on the ribonucleotide size. (5) Such structure is stereochemically possible only provided that the transcribed RNA fragment contains ribonucleotide diphosphates. As the customary RNA with a monophosphatic backbone cannot be synthesized under such conditions, formation of an intermediate referred to as the RNA transcription fragment (tf-RNA) is assumed. Advantages of the presented model over the established hypothesis of the local DNA melting during transcription are discussed.
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How to cite this article
Yu. P. Petrov, 2006. Is RNA Synthesis Possible Without Transcription Bubble Formation?. Journal of Biological Sciences, 6: 532-539.
DOI: 10.3923/jbs.2006.532.539
URL: https://scialert.net/abstract/?doi=jbs.2006.532.539
DOI: 10.3923/jbs.2006.532.539
URL: https://scialert.net/abstract/?doi=jbs.2006.532.539
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