Gemma De Vega
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Estibaliz Mateo
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Agueda Fernandez de Aranguiz
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Karmele Colom
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Rodrigo Alonso
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Aurora Fernandez-Astorga
Departamento de Inmunologia, Microbiologia y Parasitologia,
Facultad de Farmacia, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad, 7 01006 Vitoria-Gasteiz, Spain
ABSTRACT
This study was undertaken to evaluate the antimicrobial susceptibility of Campylobacter spp. strains isolated from poultry and human faeces in the Basque Country area, in the North of Spain. Poultry samples at the retail level were randomly purchased in supermarkets and human isolates were obtained from diarrhoea-infected patients. Campylobacter identification was achieved by biochemical tests and species-specific PCR. Susceptibility to seven antibiotics was determined by the agar dilution method following the NCCLS recommendations. Genotyping was carried out by PCR-RFLP of the flaA gene and PFGE profiling. Highest resistance was found for tetracycline (74%) and quinolones: nalidixic acid (76%), ciprofloxacin (72%) and enrofloxacin (61.5%). Decreasing trends in erythromycin resistance was detected in both food and human isolates. Multiresistance to three or more of the drugs was found in 4.8% strains, being more frequent in C. coli (12.5%) than in C. jejuni (2.5%).
PDF References Citation
How to cite this article
Gemma De Vega, Estibaliz Mateo, Agueda Fernandez de Aranguiz, Karmele Colom, Rodrigo Alonso and Aurora Fernandez-Astorga, 2005. Antimicrobial Susceptibility of Campylobacter jejuni and
Campylobacter coli Strains Isolated from Humans and Poultry in North of
Spain. Journal of Biological Sciences, 5: 643-647.
DOI: 10.3923/jbs.2005.643.647
URL: https://scialert.net/abstract/?doi=jbs.2005.643.647
DOI: 10.3923/jbs.2005.643.647
URL: https://scialert.net/abstract/?doi=jbs.2005.643.647
REFERENCES
- Khachatourians, G.G., 1998. Agricultural use of antibiotics and the evolution and transfer of antibiotic-resistant bacteria. Can. Med. Assoc. J., 159: 1129-1136.
Direct Link - Smith, K.E., J.M. Besser, C.W. Hedberg, F.T. Leano and J.B. Bender et al., 1999. Quinolone-resistant Campylobacter jejuni infections in Minnesota, 1992-1998. N. Engl. J. Med., 340: 1525-1532.
PubMedDirect Link - Stucki, U.R.S., J. Frey, J. Nicolet and A.P. Burnens, 1995. Identification of Campylobacter jejuni on the basis of a species-specific gene that encodes a membrane protein. J. Clin. Microbiol., 33: 855-859.
Direct Link - Gonzalez, I., K.A. Grant, P.T. Richardson, S.F. Park and M.D. Collins, 1997. Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant. J. Clin. Microbiol., 35: 759-763.
PubMedDirect Link - Nachamkin, I., K. Bohachick and C.M. Patton, 1993. Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J. Clin. Microbiol., 31: 1531-1536.
PubMedDirect Link - Cloak, O.M., G. Duffy, J.J. Sheridan, I.S. Blair and D.A. McDowell, 2001. A survey on the incidence of Campylobacter sp. and the development of a surface adhesion polymerase chain reaction (SA-PCR) assay for the detection of Campylobacter jejuni in retail meat products. Food Microbiol., 18: 287-298.
- Luber, P.J., H.H. Wagner and E. Bartelt, 2003. Antimicrobial resistance in Campylobacter jejuni and Campylobacter coli strains isolated in 1991 and 2001-2002 from poultry and humans in Berlin, Germany. Antimicrob. Agents Chemother., 47: 3825-3830.
Direct Link - Van Looveren, M., G. Daube, L. de Zutter, J.M. Dumont and C. Lammens et al., 2001. Antimicrobial susceptibilities of Campylobacter strains isolated from food animals in Belgium. J. Antimicrob. Chemother., 48: 235-240.
PubMedDirect Link - Endtz, H.P., G.I. Ruijs, B. van Klingeren, W.H. Jansen, T. van der Reyden and R.P. Mouton, 1991. Quinolone resistance in Campylobacter isolated from man and poultry following the introduction of fluoroquinolones in veterinary medicine. J. Antimicrob. Chemother., 27: 199-208.
CrossRefDirect Link