Elmer-Rico E. Mojica
Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Banos, 4031 College, Laguna, Philippines
Florinia E. Merca
Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Banos, 4031 College, Laguna, Philippines
ABSTRACT
A lectin isolated and purified by gel chromatography from the internal organs of Holothuria scabra Jaeger was assayed for different biological properties. The lectin was found to have hemolytic and mitogenic activities. It was also found to be a good inhibitor of seed germination and inhibited the germination of radish seeds. LC50 against Artemia salina is lower than commercial therapeutic agents. No antimicrobial, antiprotozoal, clastogenic, insecticidal and molluscicidal activities were observed.
PDF References Citation
How to cite this article
Elmer-Rico E. Mojica and Florinia E. Merca, 2005. Biological Properties of Lectin from Sea Cucumber (Holothuria scabra
Jaeger). Journal of Biological Sciences, 5: 472-477.
DOI: 10.3923/jbs.2005.472.477
URL: https://scialert.net/abstract/?doi=jbs.2005.472.477
DOI: 10.3923/jbs.2005.472.477
URL: https://scialert.net/abstract/?doi=jbs.2005.472.477
REFERENCES
- Goldstein, I.J., R.C. Hughes, M. Monsigny, T. Osawa and N. Sharon, 1980. What should be called a lectin? Nature, 285: 66-66.
CrossRef - Pajic, I., Z. Kljajic, N. Dogovic, D. Sladic, Z. Juranic and M.J. Gasic, 2002. A novel lectin from the sponge Haliclona cratera: Isolation, characterization and biological activity. Comp. Biochem. Physiol. C Toxicol. Pharmacol., 132: 213-221.
PubMed - Nair, S.V., M. Burandt, A. Hutchinson, R.L. Raison and D.A. Raftos, 2001. A C-type lectin from the tunicate, Styela plicata, that modulates cellular activity. Comp. Biochem. Physiol. Toxicol. Pharmacol., 129: 11-24.
PubMed - Tunkijjanukij, S. and J.A. Olafsen, 1998. Sialic acid-binding lectin with antibacterial activity from the horse mussel: Further characterization and immunolocalization. Dev. Comp. Immunol., 22: 139-150.
CrossRefPubMedDirect Link - Toyoshima, S., O. Toshiaki and A. Tonomura, 1970. Some properties of purified phytohemagglutinin from Lens culinaris seeds. Biochem. Biophy. Acta, 221: 514-521.
CrossRef - Biswas, C., D. Sinha and C. Mandal, 2000. Investigation on intraction of achatinin, a 9-O-acetylsialic acid binding lectin, with lipopolysaccharide in the innate immunity of Achatina fulica snails. Mol. Immunol., 237: 745-754.
PubMed - Ayouba, A., H. Causse, E.J.M. van Damme, W.J. Peumans, C. Cambillau and P. Rouge, 1994. Interaction of plant lectins with the components of the bacterial cell wall peptidoglycan. Biochem. Syst. Ecol., 22: 153-159.
CrossRef - Kumar, M.A., D.E. Timms, K.E. Neet, W.G. Owen and W. Peumans, 1993. Characterization of the lectin from the bulbs of Eranthis hyemalis, winter aconite as an inhibitor of protein synthesis. J. Biol. Chem., 268: 25176-25183.
Direct Link - Sequira, L. and T.L. Graham, 1977. Agglutination of avirulent strains of Pseudomonas sofanacearum by potato lectin Physiol. Pathol., 11: 43-54.
CrossRef - Putnam, A.R., 1988. Allelochemicals from plants as herbicides. Weed Technol., 2: 510-518.
Direct Link - Ryoyama, K., 1973. Studies on the biological properties of coelomic fluid of sea urchin. Biophys. Biochim. Acta, 320: 157-165.
CrossRef - Canicatti, C., 1987. Membrane damage by coelomic fluid from Holothuria polii (Echinodermata). Experientia, 43: 611-614.
PubMed - Chintala, M.M., S.E. Ford, W.S. Fisher and K.A. Ashton-Alcox, 1994. Oyster serum agglutinins and resistance to protozoan parasites. J. Shellfish Res., 13: 115-121.
Direct Link