Figen Ertan
Department of Biology, Faculty of Science and Art, Trakya University, 22080, Edime, Turkey
Filiz E. Sanal
Department of Biology, Faculty of Science and Art, Trakya University, 22080, Edime, Turkey
Aysegul C. Kaboglu
Department of Biology, Faculty of Science and Art, Trakya University, 22080, Edime, Turkey
Tulin Aktac
Department of Biology, Faculty of Science and Art, Trakya University, 22080, Edime, Turkey
Elvan Bakar
Department of Biology, Faculty of Science and Art, Trakya University, 22080, Edime, Turkey
ABSTRACT
The preparation and some biochemical properties of inulinase from R. solani which isolated from soil in Tekirdag - Turkey, was carried out by dialysis and concentrated with silica gel-60 then, further proceeded with Sephadex G-150 and DEAE-Cellulose chromatographies. The specific activity of the enzyme was enhanced from 0.256-5.43 U mg -1. The enzyme showed maximum activity at 35°C and pH 5.0, it was stable in the pH range of 5.0-6.5 and up to 40°C. The activity of enzyme was inhibited strongly by Hg2+ and Ag2+ and KCN and DTNB. The action mode of enzyme for inulin was determined as endohydrolitic-action by TLC.
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How to cite this article
Figen Ertan, Filiz E. Sanal, Aysegul C. Kaboglu, Tulin Aktac and Elvan Bakar, 2005. Some Properties of Inulinase from Rhizoctonia solani. Journal of Biological Sciences, 5: 330-334.
DOI: 10.3923/jbs.2005.330.334
URL: https://scialert.net/abstract/?doi=jbs.2005.330.334
DOI: 10.3923/jbs.2005.330.334
URL: https://scialert.net/abstract/?doi=jbs.2005.330.334
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