W. Shumi
Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh
Md. Towhid Hossain
Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh
M. N. Anwar
Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh
ABSTRACT
Following the enrichment technique in total 25 microbial colonies were isolated, purified, preserved and tested for their proteolytic ability. Among the bacterial isolate D1 showed the maximum ability to degrade egg albumin, milk casein and gelatin. On the basis of cultural, morphological, physiological and biochemical characteristics the isolate D1 was identified as Bacillus fastidiosus den Dooren de Jong. The isolate (D1) produced maximum protease at 37 ° C temperature and pH 7.0. Cellobiose (carbon source) and (NH4)2HPO4 (nitrogen source) were found to induce the protease production of the isolate and showed highest proteolytic activity at temperature 35 ° C and pH 6.5.
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How to cite this article
W. Shumi, Md. Towhid Hossain and M. N. Anwar, 2004. Proteolytic Activity of a Bacterial Isolate Bacillus fastidiosus den
Dooren de Jong. Journal of Biological Sciences, 4: 370-374.
DOI: 10.3923/jbs.2004.370.374
URL: https://scialert.net/abstract/?doi=jbs.2004.370.374
DOI: 10.3923/jbs.2004.370.374
URL: https://scialert.net/abstract/?doi=jbs.2004.370.374
REFERENCES
- Rao, M.B., A.M. Tanksale, M.S. Ghatge and V.V. Deshpande, 1998. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev., 62: 597-635.
Direct Link - Alvarez-Sanchez, M.E., L. Avila-Gonzalez, C. Becerril-Garcia, L.V. Fattel-Facenda, J. Ortega-Lopez and R. Arroyo, 2000. A novel cysteine proteinase (CP65) of Trichomonas vaginalis involved in cytotoxicity. Microbial. Pathogen., 28: 193-202.
CrossRefDirect Link - Gianna, P., B. Carmen, C. Giovanna, G. Paola, M. Gennaro, M. Maria and S. Giovanni, 2001. Purification, characterization and functional role of a novel extracellular protease from Pleurotus ostreatus. Applied Environ. Microbiol., 67: 2754-2757.
CrossRefDirect Link - Thangam, E.B. and G.S. Rajkumar, 2002. Purification and characterization of alkaline protease from Alcaligenes faecalis. Biotechnol. Applied Biochem., 35: 149-154.
Direct Link - Shumi, W., M.T. Hossain and M.N. Anwar, 2004. Isolation and purification of Aspergillus funiculosus G. Smith and its enzyme protease. Pak. J. Biol. Sci., 7: 312-317.
Direct Link - Matta, H., V. Punj and S.S. Kanwar, 1997. An immuno-dot blot assay for detection of thermostable protease from Pseudomonas sp. AFT-36 of dairy origin. Applied Microbiol., 25: 300-302.
PubMed - Henriette, C., S. Zinebi, M.F. Aumaitre, E. Petitdemange and H. Petitdemange, 1993. Protease and lipase production by a strain of Serratia marcescens. J. Ind. Microbiol., 12: 129-135.
CrossRefDirect Link - Kalisz, H.M., D. Moore and D.A. Wood, 1986. Protein utilization by basidiomycete fungi. Trans. Brit. Mycol. Soc., 86: 519-525.
Direct Link - Micales, J.A., 1992. Proteinases of the brown rot fungus Postia placenta. Mycologia, 84: 815-822.
Direct Link - Vorbeck, M.L. and J.F. Cone, 1963. Characteristics of an intracellular proteinase system of a Trichosporon species isolated from trappist-type cheese. Applied Microbiol., 11: 23-27.
Direct Link - North, M.J., 1982. Comparative biochemistry of the proteinases of eucaryotic microorganisms. Microbiol. Rev., 46: 308-340.
Direct Link