Sebastiao D. Santos-Filho
UNIFOA, Department of Biophysics, Av. Paulo Erlei Alves de Abrantes,
1325,27251-970 Volta Redonda, RJ, Brazil
Glaucio L. Dire
Department of Biophysics and Biometry, IBRAG, Rio de Janeiro State University, Av. 28 de Setembro, 87, 20551-030 Rio de Janeiro, RJ, Brazil
Elaine Lima
Department of Biophysics and Biometry, IBRAG, Rio de Janeiro State University, Av. 28 de Setembro, 87, 20551-030 Rio de Janeiro, RJ, Brazil
Marcia N. Oliveira
Department of Biophysics and Biometry, IBRAG, Rio de Janeiro State University, Av. 28 de Setembro, 87, 20551-030 Rio de Janeiro, RJ, Brazil
Mario Bernardo-Filho
Department of Biophysics and Biometry, IBRAG, Rio de Janeiro State University, Av. 28 de Setembro, 87, 20551-030 Rio de Janeiro, RJ, Brazil
ABSTRACT
The present study was carried out to investigate the possibility of Mentha crispa (Mint; M. crispa) extract being capable to alter the labeling of blood elements with Technetium-99m (99mTc). Blood was incubated with M. crispa extract. Stannous chloride solution and 99mTc, as sodium pertechnetate, were added. Blood was centrifuged and plasma (P) and blood cells (BC) were isolated. Samples of P and BC were also precipitated, centrifuged and insoluble (IF) and soluble (SF) separated. The percentage of radioactivity (%ATI) in BC, IF-P and IF-BC was calculated. Histological evaluations were performed and the morphology of the red blood cells was observed under optical microscopy showing important morphological alterations on the shape of the RBC treated with M. crispa extract. The %ATI decreased: on BC from 97.3 ± 1.92 to 60.0 ± 2.44; on IF-P from 74.8 ± 3.78 to 9.99 ± 3.61; on IF-BC from 88.6 ± 5.41 to 58.4 ± 11.55. The substances of the M. crispa extract could increase the valence of the tin ion from stannous (+2) to stannic (+4). This fact would decrease the %ATI on blood elements and would indicate the presence of oxidant agents in the M. crispa extract with possible generation of free radicals.
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How to cite this article
Sebastiao D. Santos-Filho, Glaucio L. Dire, Elaine Lima, Marcia N. Oliveira and Mario Bernardo-Filho, 2004. Effect of Mentha crispa (mint) Extract on the Labeling of Blood Elements
with Technetium-99m: A Possible Evaluation of Free Radicals. Journal of Biological Sciences, 4: 266-270.
DOI: 10.3923/jbs.2004.266.270
URL: https://scialert.net/abstract/?doi=jbs.2004.266.270
DOI: 10.3923/jbs.2004.266.270
URL: https://scialert.net/abstract/?doi=jbs.2004.266.270
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