S. Jilani
Dean Faculty of Health Management, Baqai Medical University, Karachi, Pakistan
M. Altaf Khan
Dean Faculty of Health Management, Baqai Medical University, Karachi, Pakistan
ABSTRACT
In Pakistan, insecticides are widely used in agriculture. Despite their biodegradable nature, some are highly toxic and their residues are found in the environment. Moreover, its removal from wastewater generated during manufacture becomes inevitable. Reports on the mineralization of a spectrum of different insecticides by a single potential strain are scarce. In this study, a bacterial strain was isolated from soil using enrichment technique and identified as Pseudomonas sp. by microscopic examination and biochemical tests. Growth curve experiments showed that Pseudomonas strain was able to grow in nutrient medium containing malathion (35-220 mg L-1), methamidophos (80-320 mg L-1), cartap (60-120 mg L-1) and cypermethrin (40-125 mg L-1) pesticide. However, the optimum concentration which support normal bacterial growth during 24 h was found to be 120 mg L-1 malathion, 160 mg L-1 methamidophos, 80 mg L-1 cartap and 60 mg L-1cypermethrin. When compared with the control test, a significant increased in bacterial population was noted at low concentration of each pesticide, however at high concentration lag phase increased but no zone of inhibition observed. These data indicate that the isolated Pseudomonas strain can be used as a microorganism for the bioremediation of pesticide contaminated soil or water.
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How to cite this article
S. Jilani and M. Altaf Khan, 2004. Isolation, Characterization and Growth Response of Pesticides Degrading Bacteria. Journal of Biological Sciences, 4: 15-20.
DOI: 10.3923/jbs.2004.15.20
URL: https://scialert.net/abstract/?doi=jbs.2004.15.20
DOI: 10.3923/jbs.2004.15.20
URL: https://scialert.net/abstract/?doi=jbs.2004.15.20
REFERENCES
- Bhadhade, B.J., S.S. Sarnaik and P.P. Kanekar, 2002. Bioremediation of an industrial effluent containing monocrotophos. Curr. Microbiol., 45: 346-349.
CrossRefDirect Link - El-Deeb, B.A., S.M. Soltan, A.M. Ali and K.A. Ali, 2000. Detoxication of herbicide diuron by Pseudomonas sp. Folia Microbiol., 45: 211-216.
CrossRefDirect Link - Haugland, R.A., D.J. Schlemm, R.P. Lyons, P.R. Sferra and A.M. Chakrabarty, 1990. Degradation of chlorinated phenoxy acetate herbicides 2,4-dichlorophenoxy acetic acid and 2,4,5-tricholorophenoxy acetic acid by pure and mixed culture. Applied Environ. Microbiol., 56: 1357-1362.
Direct Link - Hughes, S.M. and D.G. Cooper, 1996. Biodegradation of phenol using the self-cycling fermentation (SCF) technique. Biotechnol. Bioengg., 51: 112-119.
Direct Link - Karpouzas, D.G., J.A. Morgan and A.Walker, 2000. Isolation and characterization of 23 carbofuran-degrading bactertia from soils from distant geographical areas. Lett. Applied Microbiol., 31: 353-358.
Direct Link - Lee, S.G., B.D. Yoon, Y.H. Park and H.M. Oh, 1998. Isolation of a novel pentacholorophenol-degrading bacterium, Pseudomonas sp. Bu 34. J. Applied Microbiol., 85: 1-8.
Direct Link - Mallick, K., K. Bharati, A. Banerji, N.A. Shakil and N. Sethunathan, 1999. Bacterial degradation of chlorpyrifos in pure cultures and in soil. Bull. Environ. Contam. Toxicol., 62: 48-54.
CrossRefDirect Link - Maloney, S.E., A. Maule and A.R.W. Smith, 1988. Microbial transformation of the pyrethroid insecticides. Permethrin, deltamethrin, fastac, fenvalerate and fluvalinate. Applied Environ. Microbiol., 54: 2874-2876.
Direct Link - Martin, M., G. Mengs, E. Plaza, C. Garbi and M. Sanchez et al., 2000. Propachlor removal by Pseudomonas strain GCH1 in an immobilized cell system. Applied Environ. Microbiol., 66: 1190-1194.
Direct Link - Mercadier, C., D. Garcia, D. Vega, J. Bastide and C. Coste, 1996. Metabolism of iprodione in adapted and nonadapted soils; Effect of soil inoculation with iprodione-degrading arthrobacter strain. Soil Biol. Biochem., 28: 1791-1796.
CrossRefDirect Link - Parekh, N.R., A. Walker, S.J. Roberts and S.J. Welch, 1994. Rapid degradation of triazinone herbicide metamitron by a Rhodococcus sp. Isolated from treated soil. J. Applied Bacteriol., 77: 467-475.
Direct Link - Ralebits, T.K., E. Senior and H.W. Van Versevell, 2002. Microbial aspects of atrazine degradation in natural environments. Biodegradation, 13: 11-19.
CrossRefDirect Link - Ramanathan, M.P. and D. LailithaKumari, 1999. Complete mineralization of methyl parathion by Pseudomonas sp. A3. Applied Biochem. Biotechnol., 80: 1-12.
CrossRefDirect Link - Ramos, T.L., E. Duque, M.J. Huertas and A. Haidour, 1995. Isolation and expansion of the catabolic potential of a Pseudomonas putida straion able to grow in the presence of high concentration of aromatic hydrocarbons. J. Bacteriol., 177: 3911-3916.
Direct Link - Rani, N.L. and D. Lalithakumari, 1994. Degradation of methyl parathion by Pseudomonas putida. Can. J. Microbiol., 40: 1000-1006.
CrossRefPubMedDirect Link - Roy, D., H. Monstafa and K. Maillacheruvu, 1997. Analine degradation in a soil slurry bioreactor. J. Environ. Sci. Health, 32: 2364-2377.
Direct Link - Singh, A.K. and P.K. Seth, 1989. Degradation of malathion by microorganisms isolated from industrial effluents. Bull. Environ. Conatam. Toxicol., 43: 28-35.
CrossRefDirect Link - Zacharias, B., E. Lang and H.H. Hanert, 1995. Biodegradation of chlorinated aromatic hydro-carbons in slowsand filters simulating conditions in contaminated soil-Pilot study for in situ cleaning of an industrial site. Water Res., 29: 1663-1671.
CrossRefDirect Link - Wenk, M., T. Baumgartner, T.D. Fuchs, J. Kuesera, J. Zopfi and G. Stuchi, 1998. Rapid atrazine degrading Pseudomonas sp. Strain. Applied Microbiol. Biotechnol., 49: 624-630.
CrossRef