Shamima Nasrin
Biological Research Division, Dhaka Laboratory, BCSlR, Bangladesh
M. Monzur Hossain
Department of Botany, University of Rajshahi, Plant Tissue Culture Section
Anjumanara Khatun
Biological Research Division, Dhaka Laboratory, BCSlR, Bangladesh
M. Firoz Alam
Department of Botany, University of Rajshahi, Plant Tissue Culture Section
M. Rezaul Karim Mondal
Biological Research Division, Dhaka Laboratory, BCSlR, Bangladesh
ABSTRACT
Callus was induced from nodal internodal explants of potato Cvs. Multa and Diamant through the proper adjustment of auxin and cytokinin combinations. Callusing response was the best in MS supplemented with 1.0 mg-1 NAA + 1.0 mg-1 BA and 1.0 mg-1 NAA+ 0.5 mg-1 BA. The callus developed from both nodal and internodal segments induced to develop shoot when subculture on to MS containing same growth regulator formulations. MS containing 3 mg l-1 KIN with 1.5 mg l-1 NAA was the most responsive medium for shoot regeneration. Plants regenerated through callus culture after transplanting in field displayed somaclonal variation for plant height, number of laves/plant, number of tubers/plant and tuber weight/plant. This variability may incorporate for the potato breeding program.
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How to cite this article
Shamima Nasrin, M. Monzur Hossain, Anjumanara Khatun, M. Firoz Alam and M. Rezaul Karim Mondal, 2003. Induction and Evaluation of Somaclonal Variation in Potato (Solanum tuberosum L.). Journal of Biological Sciences, 3: 183-190.
DOI: 10.3923/jbs.2003.183.190
URL: https://scialert.net/abstract/?doi=jbs.2003.183.190
DOI: 10.3923/jbs.2003.183.190
URL: https://scialert.net/abstract/?doi=jbs.2003.183.190
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